Next Article in Journal
Calcium Orthophosphates as Bioceramics: State of the Art
Previous Article in Journal
Biocompatibility and Alkaline Phosphatase Activity of Phosphorylated Chitooligosaccharides on the Osteosarcoma MG63 Cell Line
Article Menu

Export Article

Open AccessCommunication
J. Funct. Biomater. 2010, 1(1), 14-21; doi:10.3390/jfb1010014

Autologous Cell Delivery to the Skin-Implant Interface via the Lumen of Percutaneous Devices in vitro

Department of Oral and Maxillofacial Surgery, 1150 W Medical Dr. MSRBII A560, University of Michigan, Ann Arbor, MI 48109, USA
Received: 18 October 2010 / Revised: 6 November 2010 / Accepted: 21 November 2010 / Published: 25 November 2010
View Full-Text   |   Download PDF [502 KB, uploaded 25 November 2010]   |  

Abstract

Induced tissue regeneration around percutaneous medical implants could be a useful method to prevent the failure of the medical device, especially when the epidermal seal around the implant is disrupted and the implant must be maintained over a long period of time. In this manuscript, a novel concept and technique is introduced in which autologous keratinocytes were delivered to the interfacial area of a skin-implant using the hollow interior of a fixator pin as a conduit. Full thickness human skin explants discarded from surgeries were cultured at the air-liquid interface and were punctured to fit at the bottom of hollow cylindrical stainless steel fixator pins. Autologous keratinocytes, previously extracted from the same piece of skin and cultured separately, were delivered to the specimens thorough the interior of the hollow pins. The delivered cells survived the process and resembled undifferentiated epithelium, with variations in size and shape. Viability was demonstrated by the lack of morphologic evidence of necrosis or apoptosis. Although the cells did not form organized epithelial structures, differentiation toward a keratinocyte phenotype was evident immunohistochemically. These results suggest that an adaptation of this technique could be useful for the treatment of complications arising from the contact between skin and percutaneous devices in vivo. View Full-Text
Keywords: biointegration; cell delivery; cell-material interaction biointegration; cell delivery; cell-material interaction
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Peramo, A. Autologous Cell Delivery to the Skin-Implant Interface via the Lumen of Percutaneous Devices in vitro. J. Funct. Biomater. 2010, 1, 14-21.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Funct. Biomater. EISSN 2079-4983 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top