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Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry
Department of Mechanical Engineering, University of Colorado at Boulder, Membrane Science, Engineering and Technology (MAST) Center, 427 UCB, Boulder, CO 80309–0427, USA
Genencor, a Danisco Division, 925 Page Mill Rd, Palo Alto, CA 94304, USA
Department of Civil, Environmental and Architectural Engineering, University of Colorado at Boulder, 428 UCB, Boulder, CO 80309–0428, USA
* Author to whom correspondence should be addressed.
Received: 27 May 2011; in revised form: 26 July 2011 / Accepted: 5 August 2011 / Published: 10 August 2011
Abstract: Novel signal-processing protocols were used to extend the in situ sensitivity of ultrasonic frequency-domain reflectometry (UFDR) for real-time monitoring of microfiltration (MF) membrane fouling during protein purification. Different commercial membrane materials, with a nominal pore size of 0.2 µm, were challenged using bovine serum albumin (BSA) and amylase as model proteins. Fouling induced by these proteins was observed in flat-sheet membrane filtration cells operating in a laminar cross-flow regime. The detection of membrane-associated proteins using UFDR was determined by applying rigorous statistical methodology to reflection spectra of ultrasonic signals obtained during membrane fouling. Data suggest that the total power reflected from membrane surfaces changes in response to protein fouling at concentrations as low as 14 μg/cm2, and results indicate that ultrasonic spectra can be leveraged to detect and monitor protein fouling on commercial MF membranes.
Keywords: proteins; microfiltration; fouling; ultrasonic frequency-domain reflectometry
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MDPI and ACS Style
Kujundzic, E.; Greenberg, A.R.; Fong, R.; Hernandez, M. Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry. Membranes 2011, 1, 195-216.
Kujundzic E, Greenberg AR, Fong R, Hernandez M. Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry. Membranes. 2011; 1(3):195-216.
Kujundzic, Elmira; Greenberg, Alan R.; Fong, Robin; Hernandez, Mark. 2011. "Monitoring Protein Fouling on Polymeric Membranes Using Ultrasonic Frequency-Domain Reflectometry." Membranes 1, no. 3: 195-216.