Vaccines 2013, 1(3), 225-249; doi:10.3390/vaccines1030225

Vector Design for Improved DNA Vaccine Efficacy, Safety and Production

Received: 14 May 2013; in revised form: 12 June 2013 / Accepted: 18 June 2013 / Published: 25 June 2013
(This article belongs to the Special Issue DNA Vaccines)
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Abstract: DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy.
Keywords: DNA vaccination; plasmid; antibiotic-free; non-viral; fermentation; immunization; adjuvant; innate immunity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Williams, J.A. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production. Vaccines 2013, 1, 225-249.

AMA Style

Williams JA. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production. Vaccines. 2013; 1(3):225-249.

Chicago/Turabian Style

Williams, James A. 2013. "Vector Design for Improved DNA Vaccine Efficacy, Safety and Production." Vaccines 1, no. 3: 225-249.

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