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Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
Nature Technology Corporation/Suite 103, 4701 Innovation Drive, Lincoln, NE 68521, USA
Received: 14 May 2013; in revised form: 12 June 2013 / Accepted: 18 June 2013 / Published: 25 June 2013
Abstract: DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy.
Keywords: DNA vaccination; plasmid; antibiotic-free; non-viral; fermentation; immunization; adjuvant; innate immunity
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Williams, J.A. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production. Vaccines 2013, 1, 225-249.
Williams JA. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production. Vaccines. 2013; 1(3):225-249.
Williams, James A. 2013. "Vector Design for Improved DNA Vaccine Efficacy, Safety and Production." Vaccines 1, no. 3: 225-249.