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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2014, 3(4), 263-281; doi:10.3390/microarrays3040263

Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array

1
Sime Darby Technology Centre, Universiti Putra Malaysia, 1st Floor, Block B, UPM-MTDC Technology Centre III, Lebuh Silikon, 43400 Serdang, Selangor, Malaysia
2
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SD, UK
3
School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Malaysia
4
Department of Biological Sciences, Faculty of Science, National University of Singapore, Lower Kent Ridge Road, Singapore 117543, Singapore
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 22 August 2014 / Revised: 22 September 2014 / Accepted: 3 November 2014 / Published: 13 November 2014
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Abstract

Gene expression changes that occur during mesocarp development are a major research focus in oil palm research due to the economic importance of this tissue and the relatively rapid increase in lipid content to very high levels at fruit ripeness. Here, we report the development of a transcriptome-based 105,000-probe oil palm mesocarp microarray. The expression of genes involved in fatty acid (FA) and triacylglycerol (TAG) assembly, along with the tricarboxylic acid cycle (TCA) and glycolysis pathway at 16 Weeks After Anthesis (WAA) exhibited significantly higher signals compared to those obtained from a cross-species hybridization to the Arabidopsis (p-value < 0.01), and rice (p-value < 0.01) arrays. The oil palm microarray data also showed comparable correlation of expression (r2 = 0.569, p < 0.01) throughout mesocarp development to transcriptome (RNA sequencing) data, and improved correlation over quantitative real-time PCR (qPCR) (r2 = 0.721, p < 0.01) of the same RNA samples. The results confirm the advantage of the custom microarray over commercially available arrays derived from model species. We demonstrate the utility of this custom microarray to gain a better understanding of gene expression patterns in the oil palm mesocarp that may lead to increasing future oil yield. View Full-Text
Keywords: microarray; oil palm; mesocarp; gene expression microarray; oil palm; mesocarp; gene expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Wong, Y.C.; Kwong, Q.B.; Lee, H.L.; Ong, C.K.; Mayes, S.; Chew, F.T.; Appleton, D.R.; Kulaveerasingam, H. Expression Comparison of Oil Biosynthesis Genes in Oil Palm Mesocarp Tissue Using Custom Array. Microarrays 2014, 3, 263-281.

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