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From the third issue of 2017, Microarrays has changed its name to High-Throughput.

Open AccessArticle
Microarrays 2014, 3(4), 226-244; doi:10.3390/microarrays3040226

DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin

1
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo 113-8657, Japan
2
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan
3
Kawaku Company Limited, Shimonoseki, Yamaguchi 750-0093, Japan
4
Department of Food Science and Technology, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Minato, Tokyo 108-8477, Japan
5
School of Marine Biosciences, Kitasato University, Minami, Sagamihara, Kanagawa 252-0373, Japan
These authors contributed equally to this work.
Current address: Faculty of Life and Environmental Science, Prefectural University of Hiroshima, Nanatsuka, Shobara, Hiroshima 727-0023, Japan.
*
Author to whom correspondence should be addressed.
Received: 25 August 2014 / Revised: 28 September 2014 / Accepted: 15 October 2014 / Published: 27 October 2014
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Abstract

Pufferfish accumulate tetrodotoxin (TTX) mainly in the liver and ovary. This study aims at investigating the effect of TTX accumulation in the liver of cultured specimens of torafugu Takifugu rubripes on the hepatic gene expression by microarray analysis on Day 5 after the intramuscular administration of 0.25 mg TTX/kg body weight into the caudal muscle. TTX was detected in the liver, skin and ovary in the TTX-administered individuals. The total amount of TTX accumulated in the body was 67 ± 8% of the administered dose on Day 5. Compared with the buffer-administered control group, a total of 59 genes were significantly upregulated more than two-fold in the TTX-administered group, including those encoding chymotrypsin-like elastase family member 2A, transmembrane protein 168 and Rho GTP-activating protein 29. In contrast, a total of 427 genes were downregulated by TTX administration, including those encoding elongation factor G2, R-spondin-3, nuclear receptor activator 2 and fatty acyl-CoA hydrolase precursor. In conclusion, our results demonstrate that the intramuscular administration of TTX changes the expression of hepatic genes involved in various signaling pathways. View Full-Text
Keywords: tetrodotoxin; pufferfish Takifugu rubripes; microarray analysis; gene expression; intramuscular administration; accumulation; toxification tetrodotoxin; pufferfish Takifugu rubripes; microarray analysis; gene expression; intramuscular administration; accumulation; toxification
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Matsumoto, T.; Feroudj, H.; Kikuchi, R.; Kawana, Y.; Kondo, H.; Hirono, I.; Mochizuki, T.; Nagashima, Y.; Kaneko, G.; Ushio, H.; Kodama, M.; Watabe, S. DNA Microarray Analysis on the Genes Differentially Expressed in the Liver of the Pufferfish, Takifugu rubripes, Following an Intramuscular Administration of Tetrodotoxin. Microarrays 2014, 3, 226-244.

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