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Microarrays 2012, 1(3), 107-124; doi:10.3390/microarrays1030107

Integrated Amplification Microarrays for Infectious Disease Diagnostics

1
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA
2
Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA
*
Author to whom correspondence should be addressed.
Received: 24 September 2012 / Revised: 31 October 2012 / Accepted: 7 November 2012 / Published: 9 November 2012
(This article belongs to the Special Issue Feature Papers)
View Full-Text   |   Download PDF [458 KB, uploaded 9 November 2012]   |  

Abstract

This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. View Full-Text
Keywords: microfluidics; diagnostics; gel element arrays; asymmetric PCR; RT-PCR; reverse transcriptase; multiplex; integrated microarrays microfluidics; diagnostics; gel element arrays; asymmetric PCR; RT-PCR; reverse transcriptase; multiplex; integrated microarrays
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chandler, D.P.; Bryant, L.; Griesemer, S.B.; Gu, R.; Knickerbocker, C.; Kukhtin, A.; Parker, J.; Zimmerman, C.; George, K.S.; Cooney, C.G. Integrated Amplification Microarrays for Infectious Disease Diagnostics. Microarrays 2012, 1, 107-124.

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