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Integrated Amplification Microarrays for Infectious Disease Diagnostics
Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA
Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA
* Author to whom correspondence should be addressed.
Received: 24 September 2012; in revised form: 31 October 2012 / Accepted: 7 November 2012 / Published: 9 November 2012
Abstract: This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.
Keywords: microfluidics; diagnostics; gel element arrays; asymmetric PCR; RT-PCR; reverse transcriptase; multiplex; integrated microarrays
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Chandler, D.P.; Bryant, L.; Griesemer, S.B.; Gu, R.; Knickerbocker, C.; Kukhtin, A.; Parker, J.; Zimmerman, C.; George, K.S.; Cooney, C.G. Integrated Amplification Microarrays for Infectious Disease Diagnostics. Microarrays 2012, 1, 107-124.
Chandler DP, Bryant L, Griesemer SB, Gu R, Knickerbocker C, Kukhtin A, Parker J, Zimmerman C, George KS, Cooney CG. Integrated Amplification Microarrays for Infectious Disease Diagnostics. Microarrays. 2012; 1(3):107-124.
Chandler, Darrell P.; Bryant, Lexi; Griesemer, Sara B.; Gu, Rui; Knickerbocker, Christopher; Kukhtin, Alexander; Parker, Jennifer; Zimmerman, Cynthia; George, Kirsten S.; Cooney, Christopher G. 2012. "Integrated Amplification Microarrays for Infectious Disease Diagnostics." Microarrays 1, no. 3: 107-124.