Next Article in Journal
Repeater Insertion for Multi-Walled Carbon Nanotube Interconnects
Next Article in Special Issue
A 3D Printed Membrane-Based Gas Microflow Regulator for On-Chip Cell Culture
Previous Article in Journal
Object Tracking with LiDAR: Monitoring Taxiing and Landing Aircraft
Previous Article in Special Issue
Gelatin-Enabled Microsensor for Pancreatic Trypsin Sensing
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Appl. Sci. 2018, 8(2), 235; https://doi.org/10.3390/app8020235

Large Scale Imaging by Fine Spatial Alignment of Multi-Scanning Data with Gel Cube Device

1
NanoSquare Research Institute, Osaka Prefecture University, Osaka 599-8570, Japan
2
Department of Bioscience and Informatics, Osaka Prefecture University, Osaka 599-8570, Japan
3
Department of Biological Functions Engineering, Kyushu Institute of Technology, Kitakyushu 808-0196, Japan
*
Author to whom correspondence should be addressed.
Received: 10 January 2018 / Revised: 31 January 2018 / Accepted: 1 February 2018 / Published: 4 February 2018
(This article belongs to the Special Issue Microfluidics in Biology and Medicine)
View Full-Text   |   Download PDF [3031 KB, uploaded 4 February 2018]   |  

Abstract

In vitro three-dimensional (3D) culturing is considered essential in many biological fields. However, the imaging of developed 3D formations is often difficult, especially if the size of the sample is relatively large. The z-resolution of fluorescent imaging is low using low magnification lenses (4× and 10×) due to large focal depths. This paper describes 3D culture platform enabling large scale 3D imaging by fine spatial alignment of the image dataset obtained from multiple directions. A gel cube device was employed to conduct the multi-scanning and then a self-fluorescent microstructure in a cubic frame allows us spatially align image dataset within a few pixels. By synthesizing data from multiple scans, the platform enables us to visualize millimeter-sized 3D sample structure and individual cellular actin filaments at the same time. Millimeter depth imaging of a developed bronchial tree was achieved with high z-resolution. The device, which is applicable to most microscopy systems, can enhance the image quality without modifying current systems. View Full-Text
Keywords: 3D culture; 3D image; multi-directional imaging 3D culture; 3D image; multi-directional imaging
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Share & Cite This Article

MDPI and ACS Style

Hagiwara, M.; Nobata, R.; Kawahara, T. Large Scale Imaging by Fine Spatial Alignment of Multi-Scanning Data with Gel Cube Device. Appl. Sci. 2018, 8, 235.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top