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Appl. Sci. 2017, 7(3), 195; doi:10.3390/app7030195

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

1
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
2
Chemical Biology Center, Lishui Institute of Agricultural Sciences, Lishui 323000, Zhejiang, China
3
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
4
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
*
Authors to whom correspondence should be addressed.
Academic Editor: Chih-Ching Huang
Received: 16 November 2016 / Revised: 25 January 2017 / Accepted: 10 February 2017 / Published: 23 February 2017
View Full-Text   |   Download PDF [1415 KB, uploaded 23 February 2017]   |  

Abstract

Traditional She medicine is part of China’s cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ) tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine. View Full-Text
Keywords: traditional She medicine; Shi-Liang tea; Chimonanthus; ITS2; identification traditional She medicine; Shi-Liang tea; Chimonanthus; ITS2; identification
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Ma, S.; Lv, Q.; Zhou, H.; Fang, J.; Cheng, W.; Jiang, C.; Cheng, K.; Yao, H. Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode. Appl. Sci. 2017, 7, 195.

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