Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip
AbstractHelicobacter pylori is a spiral-shaped bacterium that grows in the human digestive tract; it infects ~50% of the global population. H. pylori induce inflammation, gastroenteritis, and ulcers, which is associated with significant morbidity and may be linked to stomach cancer in certain individuals. Motility is an essential virulence factor for H. pylori, allowing it to migrate toward and invade the epithelial lining of the stomach to shelter it from the harsh environment of the stomach. H. pylori senses pH gradients and use polar flagella to move towards the epithelium where the pH approaches neutrality. However, its chemotaxis behaviors are incompletely understood. Previous in vitro tests examining the response of H. pylori to chemical gradients have been subjected to substantial limitations. To more accurately mimic/modulate the cellular microenvironment, a nanoporous microfluidic device was used to monitor the real time chemotactic activity of single cell of H. pylori in response to urea. The results showed that microfluidic method is a promising alternative for accurate studying of chemotactic behavior of H. pylori, the application of which may also be extended in the studies of other bacteria. View Full-Text
- Supplementary File 1:
ZIP-Document (ZIP, 13196 KB)
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Weng, X.; Neethirajan, S.; Vogt, A. Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip. Appl. Sci. 2016, 6, 139.
Weng X, Neethirajan S, Vogt A. Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip. Applied Sciences. 2016; 6(5):139.Chicago/Turabian Style
Weng, Xuan; Neethirajan, Suresh; Vogt, Adam. 2016. "Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip." Appl. Sci. 6, no. 5: 139.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.