Next Article in Journal
Indoor Localization Using Semi-Supervised Manifold Alignment with Dimension Expansion
Next Article in Special Issue
Physicochemical Properties and In Vitro Dissolution of Spiramycin Microparticles Using the Homogenate-Antisolvent Precipitation Process
Previous Article in Journal
Novel Predictive Model of the Debonding Strength for Masonry Members Retrofitted with FRP
Previous Article in Special Issue
Single Cell Chemotactic Responses of Helicobacter pylori to Urea in a Microfluidic Chip
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(11), 336; doi:10.3390/app6110336

Intrant ELISA: A Novel Approach to Fabrication of Electrospun Fiber Mat-Assisted Biosensor Platforms and Their Integration within Standard Analytical Well Plates

1
Department of Electrical Engineering, School of Engineering and Sciences, Tecnologico de Monterrey, Monterrey 2501, Mexico
2
Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
3
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia
4
School of Material Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore
5
Department of Biomedical Engineering, University of California, Irvine, CA 92697, USA
6
Department of Mechanical and Aerospace Engineering, University of California, Irvine, CA 92697, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Donglei Fan
Received: 8 September 2016 / Revised: 24 October 2016 / Accepted: 24 October 2016 / Published: 5 November 2016
(This article belongs to the Special Issue Nanomanufacturing of Biomedical Systems and Devices)
View Full-Text   |   Download PDF [4764 KB, uploaded 17 November 2016]   |  

Abstract

A combination of far-field electrospinning (FFES) and free-radical polymerization has been used to fabricate coated electrospun polymer fiber mats as a new type of biosensor platform. Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) electrospun fibers were dip-coated with different compositions of poly methyl methacrylate-co-methacrylic acid (poly(MMA-co-MAA)). This synergistic approach utilizes large specific surface area of PHBV fibers and co-polymer coatings that feature an optimum concentration of surface carboxyl (–COOH) groups. The platform surface morphology, porosity and tunable hydrophobicity enhance biomolecular interactions via plurality of molecular forces. These customized fiber mats have been integrated into a newly designed 96-well plate called an “intrant enzyme-linked immunosorbent assay” or i-ELISA. I-ELISA allows colorimetric sandwich assay to be carried out without any modifications or additional steps in ELISA methodology. By introducing the fiber mats in fabrication of i-ELISA via extensions on the lid, we address some of the limitations of the previous designs while demonstrating an enhanced signal intensity up to 12 times higher than that of conventional assays. With improved sensitivity, specificity and accuracy in the detection of dengue virus, i-ELISA has proven to be a reliable platform for biomolecular recognition. The proposed fiber mat-assisted well plate in this study holds great potential as a universal approach for integration of different types of fiber mats with pre-designed specific properties in order to enhance the detection sensitivity of the assay. View Full-Text
Keywords: Bio-analytical assay; electrospun fiber mats; fiber-based analytical platform; protein immobilization; surface functional groups Bio-analytical assay; electrospun fiber mats; fiber-based analytical platform; protein immobilization; surface functional groups
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Hosseini, S.; Azari, P.; Aeinehvand, M.M.; Rothan, H.A.; Djordjevic, I.; Martinez-Chapa, S.O.; Madou, M.J. Intrant ELISA: A Novel Approach to Fabrication of Electrospun Fiber Mat-Assisted Biosensor Platforms and Their Integration within Standard Analytical Well Plates. Appl. Sci. 2016, 6, 336.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top