Pathogens 2014, 3(2), 417-436; doi:10.3390/pathogens3020417
Article

Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments

1,* email, 1,2email, 1,2email, 1email, 1,2email and 1,2,3,* email
Received: 1 March 2014; in revised form: 30 April 2014 / Accepted: 4 May 2014 / Published: 9 May 2014
(This article belongs to the Special Issue Bacterial Pathogenomics: From Technology to Application)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Vaccination with Salmonella enterica serovar Typhimurium lacking DNA adenine methyltransferase confers cross-protective immunity against multiple Salmonella serotypes. The mechanistic basis is thought to be associated with the de-repression of genes that are tightly regulated when transiting from one microenvironment to another. This de-repression provides a potential means for the production of a more highly expressed and stable antigenic repertoire capable of inducing cross-protective immune responses. To identify genes encoding proteins that may contribute to cross-protective immunity, we used a Salmonella Typhimurium DNA adenine methyltransferase mutant strain (UK-1 dam mutant) derived from the parental UK-1 strain, and assessed the transcriptional profile of the UK-1 dam mutant and UK-1 strain grown under conditions that simulate the intestinal or endosomal microenvironments encountered during the infective process. As expected, the transcriptional profile of the UK-1 dam mutant identified a set of genes more transcriptionally active when compared directly to UK-1, and stably transcribed in biologically relevant culture conditions. Further, 22% of these genes were more highly transcribed in comparison to two other clinically-relevant Salmonella serovars. The strategy employed here helps to identify potentially conserved proteins produced by the UK-1 dam mutant that stimulate and/or modulate the development of cross-protective immune responses toward multiple Salmonella serotypes.
Keywords: Salmonella Typhimurium; DNA adenine methyltransferase; transcriptome; immunity; bacteriophage; fimbriae
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MDPI and ACS Style

Miller, C.B.; Pierlé, S.A.; Brayton, K.A.; Ochoa, J.N.; Shah, D.H.; Lahmers, K.K. Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments. Pathogens 2014, 3, 417-436.

AMA Style

Miller CB, Pierlé SA, Brayton KA, Ochoa JN, Shah DH, Lahmers KK. Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments. Pathogens. 2014; 3(2):417-436.

Chicago/Turabian Style

Miller, Claire B.; Pierlé, Sebastian A.; Brayton, Kelly A.; Ochoa, Jennine N.; Shah, Devendra H.; Lahmers, Kevin K. 2014. "Transcriptional Profiling of a Cross-Protective Salmonella enterica serovar Typhimurium UK-1 dam Mutant Identifies a Set of Genes More Transcriptionally Active Compared to Wild-Type, and Stably Transcribed across Biologically Relevant Microenvironments." Pathogens 3, no. 2: 417-436.

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