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Insects 2018, 9(3), 103; https://doi.org/10.3390/insects9030103

Oviplate: A Convenient and Space-Saving Method to Perform Individual Oviposition Assays in Aedes aegypti

Laboratório de Mosquitos Geneticamente Modificados, Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo 05508-000, Brazil
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Received: 3 July 2018 / Revised: 31 July 2018 / Accepted: 9 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Integrative Mosquito Biology: From Molecules to Ecosystems)
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Abstract

Aedes aegypti is the principal vector of the urban arboviruses and the blood ingestion is important to produce the eggs in this species. To analyze the egg production in Ae. aegypti, researchers frequently use small cages or Drosophila vials to collect eggs from gravid females. Although it is affordable, the setup is time- and space-consuming, mainly when many mosquitoes need to be individually analyzed. This study presents an easy, cheap, and space-saving method to perform individual oviposition assays in Ae. aegypti using cell culture plates. This new method to access fecundity rate was named “oviplate”. The oviplates are setup with 12- or 24-well plates, distilled water and filter paper and they are 78 to 88% cheaper than the traditional Drosophila vial assay, respectively. Furthermore, to allocate 72 vitellogenic females in an insectary using Drosophila vial is necessary 4100 cm3 against 1400 cm3 and 700 cm3 when using 12- and 24-well plates, respectively. No statistical differences were found between the number of eggs laid in Drosophila vials and the oviplates, validating the method. The oviplate method is an affordable, and time- and space-efficient device, and it is simpler to perform individual fecundity analyses in Ae. aegypti. View Full-Text
Keywords: Aedes aegypti; oviposition; oviplate; fecundity assay; eggs; mosquito Aedes aegypti; oviposition; oviplate; fecundity assay; eggs; mosquito
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Ioshino, R.S.; Carvalho, D.O.; Marques, I.C.S.; Fernandes, E.S.; Capurro, M.L.; Costa-da-Silva, A.L. Oviplate: A Convenient and Space-Saving Method to Perform Individual Oviposition Assays in Aedes aegypti. Insects 2018, 9, 103.

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