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Insects 2014, 5(3), 528-538; doi:10.3390/insects5030528
Article

Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s

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Penn State Erie, The Behrend College, 4701 College Dr, Erie, PA 16563, USA
* Authors to whom correspondence should be addressed.
Received: 24 May 2014 / Revised: 20 June 2014 / Accepted: 20 June 2014 / Published: 3 July 2014
(This article belongs to the Special Issue Insect Conservation and Diversity)
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Abstract

Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.
Keywords: Diaspididae; armored scale insects; phylogenetics; molecular barcoding; invasive species; cryptic species Diaspididae; armored scale insects; phylogenetics; molecular barcoding; invasive species; cryptic species
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Campbell, A.M.; Lawrence, A.J.; Hudspath, C.B.; Gruwell, M.E. Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s. Insects 2014, 5, 528-538.

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