Insects 2014, 5(3), 528-538; doi:10.3390/insects5030528
Article

Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s

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Received: 24 May 2014; in revised form: 20 June 2014 / Accepted: 20 June 2014 / Published: 3 July 2014
(This article belongs to the Special Issue Insect Conservation and Diversity)
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Abstract: Armored scale insects pose a serious threat to habitat conservation across the globe because they include some of the most potent invasive species in the world. They are such a serious concern because their basic morphology, small size, and polyphagous feeding habits often allow them to exist undetected by growers and quarantine experts. In order to provide a potential solution to the problem, we have attempted to elucidate the effectiveness of molecular identification techniques using ribosomal 28s and endosymbiotic 16s rRNA. Sequence data was obtained from many field-collected insects to test the feasibility of identification techniques. A protocol for quick species determination based on sequence data is provided.
Keywords: Diaspididae; armored scale insects; phylogenetics; molecular barcoding; invasive species; cryptic species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Campbell, A.M.; Lawrence, A.J.; Hudspath, C.B.; Gruwell, M.E. Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s. Insects 2014, 5, 528-538.

AMA Style

Campbell AM, Lawrence AJ, Hudspath CB, Gruwell ME. Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s. Insects. 2014; 5(3):528-538.

Chicago/Turabian Style

Campbell, Alexander M.; Lawrence, Andrew J.; Hudspath, Caleb B.; Gruwell, Matthew E. 2014. "Molecular Identification of Diaspididae and Elucidation of Non-Native Species Using the Genes 28s and 16s." Insects 5, no. 3: 528-538.

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