- freely available
Insects 2012, 3(1), 307-323; doi:10.3390/insects3010307
Published: 16 March 2012
Abstract: We present a new perspective for the role of Termitomyces fungi in the mutualism with fungus-growing termites. According to the predominant view, this mutualism is as an example of agriculture with termites as farmers of a domesticated fungus crop, which is used for degradation of plant-material and production of fungal biomass. However, a detailed study of the literature indicates that the termites might as well be envisioned as domesticates of the fungus. According to the “ruminant hypothesis” proposed here, termite workers, by consuming asexual fruiting bodies not only harvest asexual spores, but also lignocellulolytic enzymes, which they mix with foraged plant material and enzymes of termite and possibly bacterial origin. This mixture is the building material of the fungus garden and facilitates efficient degradation of plant material. The fungus garden thus functions as an external rumen for termites and primarily the fungi themselves benefit from their own, and gut-derived, lignocellulolytic enzymes, using the termites to efficiently mix these with their growth substrate. Only secondarily the termites benefit, when they consume the degraded, nitrogen-enriched plant-fungus mixture a second time. We propose that the details of substrate use, and the degree of complementarity and redundancy among enzymes in food processing, determine selection of horizontally transmitted fungal symbionts at the start of a colony: by testing spores on a specific, mechanically and enzymatically pre-treated growth substrate, the termite host has the opportunity to select specific fungal symbionts. Potentially, the gut-microbiota thus influence host-fungus specificity, and the selection of specific fungal strains at the start of a new colony. We argue that we need to expand the current bipartite insect-biased view of the mutualism of fungus-growing termites and include the possible role of bacteria and the benefit for the fungi to fully understand the division of labor among partners in substrate degradation.
Mutualistic symbiosis has played a major role in evolution. Through synergy of combined abilities, a mutualistic alliance between two or more species can adapt faster than individual organisms . For example, a symbiosis between a proto-eukaryotic cell and endosymbiotic prokaryotes is at the basis of eukaryotes, which eventually led to protists, plants, fungi and animals . Another example is the symbiosis between plants and arbuscular mycorrhiza, which allowed the colonization of land by plants, fundamentally altering environmental conditions on earth .
Insects represent the most diverse animal group in the terrestrial ecosystem. Over one million species of insects have been described, but current estimates of total insect diversity vary from 3–80 million species [4,5,6]. Although most adaptive radiations of insects are likely to have been driven by flight (i.e., efficient dispersal), resistance to desiccation, voltinism and metabolic adaptations to herbivory, their capacity to engage in symbiosis may have contributed to their enormous evolutionary success . It is estimated that 20% of all insects are obligatorily associated with symbiotic microorganisms, such as endosymbiotic bacteria and gut bacteria, allowing these groups to specialize on imbalanced food sources [7,8]. The best characterized associations are with bacteria that provide nutritional benefits to their host, such as the association between aphids and Buchnera bacteria [9,10]. Wolbachia spp. are best known for their manipulation of host reproduction , but recent work has shown that they sometimes also offer protection against parasitism and pathogens . In particular, insects that feed on incomplete specialized diets, such as plant sap, vertebrate blood or woody materials, tend to engage in symbioses with partners that provide essential nutrients and/or help them in food digestion.
In insect mutualisms with fungi, the insects generally consume their symbionts as a food source, and as a source of enzymes for degradation of plant material. Behaviorally complex systems of such insect agriculture have only evolved in three insect orders: once in termites , once in ants  and seven times in ambrosia beetles . In this paper, we discuss recent work on fungus-growing termites. We focus on the unresolved debate about the exact role in substrate degradation of the symbiotic fungus. We propose a new hypothesis for the division of labor between termites and fungi in (ligno)cellulose degradation. We also discuss the potential role of bacteria, which have been studied in much less detail, and their potential to mediate host-fungus specificity.
2. The Mutualism between Macrotermitine Termites and Termitomyces Fungi
Termites of the subfamily Macrotermitinae (Termitidae) live in an obligate mutualistic symbiosis with fungi of the genus Termitomyces (basidiomycetes). Fungus-growing termites are restricted to the Old-World tropics, with the highest diversity in African tropical rain forests. This habitat has also been reconstructed as the centre of origin , likely just before the expansion of the savannah, about 31 Ma . Entering the symbiosis has allowed the fungi to overcome highly unfavorable seasonal conditions (e.g., temperature fluctuations and low moisture), and the termites to exploit complex plant substrates (e.g., lignocellulose). The current habitat of fungus-growing termites ranges from rain forest to savannah. Whereas species numbers of fungus-growing termites are highest in rainforest habitats [17,18], their relative contribution to ecosystem decomposition is highest in savannahs, with up to 20% of all carbon mineralization attributed to fungus-growing termites . The highest estimate on their contribution to carbon mineralization, 90%, relates to their activity in a very arid habitat . The transition to fungiculture in both partners has occurred only once, with no reversals to free-living states of any of the two partners .
Fungus-growing termites are eusocial insects, with division of labor among colony members in brood care and reproduction, and overlap of adult generations . Colonies of fungus-growing termites are normally founded by a single reproductive pair (Figure 1b). When the workers of the first brood are mature they leave to forage and usually acquire a specific fungus from the environment .
Presumably, along with their foraged plant material, they will collect basidiospores, which will be the inoculum of the new fungus garden. The majority of species have this horizontal transmission, implying that reproduction and dispersal of the two partners occurs independently . However, two independent transitions have occurred towards vertical symbiont transmission (i.e., where Termitomyces vegetative spores are transported in the gut of alates from a parental colony and used to inoculate the fungus comb of the newly founded colonies). In both cases, vertical transmission is uniparental, but via different sexes: via the female alate in all studied species of the genus Microtermes and via the male alate in the species Macrotermes bellicosus [13,26,27,28,29].
The symbiotic fungus grows as a single-strain monoculture [24,30] on a special substrate, the fungus comb, maintained by the termites through continuous addition of pre-digested plant material (Figure 1a,c,d). The consumption of comb material varies between species. In some species, there is a continuous turnover of comb material, with primary feces (including fungal inoculum) being deposited on top and the older comb material at the bottom being consumed (e.g., species within Macrotermes and Odontotermes). In other species, for example in species of the genus Pseudacanthotermes, the entire comb is consumed before building a new one in an empty chamber .
Fungus-growing termites show moderate specificity with their symbionts: monophyletic groups of the termites (i.e., usually either a single genus or several genera) are associated with monophyletic groups of fungi [13,32]. However, there are also signatures of host switching among certain divergent species over evolutionary time, so that termite species can be associated with different clades of fungal symbionts [17,33,34,35]. This is consistent with horizontal symbiont transmission as the ancestral and still most common transmission mode in this symbiosis (Figure 1f).
3. Large Differences in Host-Fungus Specificity
Although strict vertical transmission should lead to a high degree of host specificity, the fungal symbionts of species of fungus-growing termites with this transmission mode (Figure 1g) are not monophyletic [13,29,32,35]. Therefore, occasional events of horizontal transmission must be inferred for these species. Calibrated phylogenetic reconstructions show that species of the genus Microtermes are associated with symbionts of a relatively recent origin (about twice as young as the host genus) and one of the symbionts associated with Microtermes is highly successful and has dispersed across multiple divergent genera . Macrotermes bellicosus is associated with at least four symbiont lineages, which do not constitute a clade [13,29]. Furthermore, we have recently demonstrated that the pool of fungal symbionts reared and transmitted by M. bellicosus is not genetically isolated from the Termitomyces pool of other Macrotermes species and that occasional recombination occurs among the fungal symbionts of M. bellicosus. Thus, although the population structure of M. bellicosus fungi has a clear signature of clonal propagation, occasionally recombination and exchange with fungi associated with other Macrotermes species occurs . In contrast, high specificity is sometimes found in lineages with horizontal transmission. For example, all colonies of the fungus-growing termite Macrotermes natalensis studied so far are associated with a single, sexually reproducing, horizontally transmitted lineage of Termitomyces symbionts [33,36].
Transmission mode, therefore, does not explain observed host-fungus specificity patterns very well. Therefore, interaction specificity must depend on other factors than transmission mode. One such factor may be the role of the fungal symbiont.
4. Hypotheses on the Role of the Symbiotic Fungus
Fungus-growing termites mainly feed on dead plant material, that is to say they mostly ingest lignocellulose, the major component of plant cell walls (Figure 2). The ratios between various constituents of plant cell walls vary between plant species, but even within a single species they may also vary with age, stage of growth, and other conditions. However, cellulose is usually the dominant structural polysaccharide of plant cell walls (35–50%), followed by hemicellulose (20–35%) and lignin (10–25%)  with the cellulose and hemicellulose polymers tightly bound to the lignin . These substances are recalcitrant, and many different enzymes are involved in their degradation.
The high efficiency of fungus-growing termites in digesting these plant substances has been attributed to their symbiosis with fungi. However, the exact role of the fungal symbiont is still debated. Overall, the proposed functions fall into the following categories, which are not mutually exclusive [22,39,40,41,42]:
Termitomyces has a role in lignin degradation, which enhances the digestibility of the cellulose (proposed by Grassé and Noirot );
Termitomyces is an additional protein-rich food source (mainly the fungal nodules);
Although understanding the role of the symbiotic fungus, and variation therein, is crucial to understand the evolution and stability of this mutualism, only few studies have addressed this question. Most studies have focused on the role of the fungus in cellulose digestion for the termites, and are mainly based on species of the genus Macrotermes. Moreover, these studies are difficult to compare because they had different foci, were based on different species and/or used critically different methodologies. A complicating factor is that the role of the fungus differs between genera and species [31,45,46,47], and may even vary among symbiont strains within species (TN, unpublished data).
Physical disruption of the lignocellulose by insect mastication and chemical disruption of the lignin aids the access of cellulases to the cellulose substrate. Hyodo and co-workers  found evidence for the lignin hypothesis, originally proposed by Grassé and Noirot , based on chemical composition of fungus combs of Macrotermes annandalei, M. gilvus , M. carbonarius and M. muelleri. Rohrmann , also showed a decreasing lignin content in old fungus comb of Macrotermes ukuzii and M. natalensis, consistent with a role in lignin degradation for the fungal symbionts of these species.
However, lignin degradation does not seem to be the main role of the symbiotic fungi in all species of fungus-growing termites. For example, lignin degradation was shown to be rather inefficient  in Odontotermes -O. sp.1, O. formosanus, O. takensis and H. makhamensis (belonging to the Asiatic genus Hypotermes derived from Odontotermes; )- Ancistrotermes pakistanicus and Pseudacanthotermes militaris.
Next to the capabilities to degrade lignin attributed to Termitomyces associated with Macrotermes , cellulolytic and xylanolytic activities have also been ascribed to Termitomyces. Termitomyces nodules associated with Macrotermes subhyalinus contain specific cellulase activity . Likewise, Termitomyces strains associated with M. bellicosus and M. mulleri were shown to have cellulolytic activity [31,45,51]. However, Veivers and co-workers  found that the amount of enzyme produced by the symbiotic fungi associated with M. michaelseni and M. subhyalinus was not sufficient to account for the measured respiration of the workers. Some studies have shown xylanolytic activity of Termitomyces associated with M. bellicosus [45,52] and endo- and exoxylanases have been isolated from the fungal symbiont of this host . Also from the Termitomyces sp. associated with M. subhyalinus endoxylanases have been isolated and purified .
In contrast, in Odontotermes formosanus, cellulase activity has been found to be significantly higher in workers than in the symbiotic fungus  and the termites themselves seem to be able to excrete cellulase . From the synergy of the different cellulases, a higher activity efficiency in degrading the substrate could emerge, although the low cellulase activity found in the fungus suggests that the main role of the cultivated fungus for this species is not in cellulase degradation. The fungus Termitomyces clypeatus (hosted by O. lacustris  but likely also with other Odontotermes species—see phylogenetic reconstruction in ), has been shown to be able to produce a broad spectrum of extracellular glucosidases (cellulase, sucrase, cellobiase etc., ), but also of xylanases [58,59] and laccases .
Martin and Martin, studying substrate degradation in M. natalensis, proposed the acquired enzymes hypothesis (Figure 3a) . According to this hypothesis, Termitomyces provides cellulases and xylanases, which complement (or work synergistically with) the endogenous enzymes in the termite gut. Consistent with this hypothesis, high concentrations of fungal enzymes were found in other species of Macrotermes [45,50,51]. However, in M. michaelseni and M.bayerni, enzymes of fungal origin were scarcely available in the guts of termite workers suggesting that most cellulolytic enzyme activity was of termite origin [41,61,62]. Furthermore, a division of labor between termite workers has been demonstrated in some species [63,64], with younger workers consuming nodules and tending the comb structure and older workers feeding on degraded fungus comb and foraging for plant material. Therefore, termite workers only have access to the fungal enzymes for a limited period of their life.
This criticism also applies to the hypothesis that Termitomyces is an additional protein-rich food source, mainly in the form of nodules. However, it could be imagined that growing younger workers need more protein-rich diets (Figure 3b). For Ancistrotermes pakistanicus and Pseudacanthotermes militaris , and for Ancistotermes cavitorax , where no lignin degradation capabilities have been demonstrated, the main role of the fungus was suggested to be a protein-rich food source. Next to a protein-rich food source, it is now well established that nodules also deliver spores, which can survive gut passage, for the inoculation of new comb fragments .
5. The Ruminant Hypothesis: Termites Helping the Fungus to Degrade Lignocellulose
Surprisingly, none of the current hypotheses explicitly addresses the question of fungal growth itself, and the growth substrate for the fungus. Instead, the fungus is often seen as an enzyme factory, used by the termites to accomplish cellulose degradation inside the termite gut. This view is probably influenced by the situation in other groups of termites, where the degradation of cellulose is achieved by gut bacteria and protozoa.
Here, we explicitly consider the advantage for both partners in the mutualism and propose a new hypothesis, the ruminant hypothesis. As first described by Swift , in an external rumen animals assimilate or digest the metabolic products of microflora associated with decomposing plant material. According to this view, the fungus garden would function as an external rumen for termites. Collins  already in 1983 described the symbiosis with the fungus as a way for termites to have an “external digestive system”, which decomposes their feces, decreasing the C/N ratio of foraged products by metabolizing carbohydrates prior to consumption.
It is now well-established that nodules deliver asexual spores used to inoculate fresh garden substrate [18,58]. Furthermore, it has been demonstrated for several species that the nodules contain enhanced concentrations of lingo-cellulolytic enzymes [44,67,68]. We propose that the first gut passage serves to mix fungal enzymes and spores with the substrate and with enzymes of termite and possibly bacterial origin (Figure 3c). This mixture facilitates efficient degradation of plant material. This means that the fungus uses the termites to efficiently spread ligno-cellulolytic enzymes, both fungus and insect derived (and putatively bacterial; see below).
An important prediction of this model is that enzymes are not secreted in large amounts by the mycelium, but accumulate in the nodules and become available upon partial digestion of cells during gut passage. Obviously, the ruminant hypothesis has a clear parallel with the acquired enzyme hypothesis [44,67]. However, a crucial difference between the two is the moment the termites benefit. According to the acquired enzyme hypothesis, there is a direct benefit of fungal-derived enzymes, which accomplish intestinal cellulose breakdown, thus making nutrients available for the termites. In contrast, according to the ruminant hypothesis, initially the fungi benefit from their own enzymes and from termite acquired enzymes using the termites to efficiently mix these enzymes with the substrate and their own asexual spores. The termites only secondarily profit from these enzymes in the form of degraded, nitrogen-enriched plant material and fungal biomass, when old fragments of the comb are consumed. The two main lines of criticism against the acquired enzyme hypothesis, viz. that the amounts of enzyme produced by the fungus are not sufficient, and that fungal growth itself is not explicitly considered, thus do not apply to the ruminant hypothesis.
Since the nodule, which presumably descends from one or a few cells, produces both spores and enzymes, this implies a strong link between phenotype (enzyme production) and genotype. This genotype-phenotype coupling should additionally provide a better protection against cheaters not producing enzyme, compared to excretion of enzymes by the mycelium as a common good, of which other genotypes can also profit.
6. Other Putative Nutritional Symbionts
It is well established that the Termitomyces symbiont contributes, in one way or another, to plant material decomposition, but as the host termites also produce their own cellulases, the exact division of labor between the two partners remains to be established. It has been assumed that the strongly aerobic fungal symbiont removes the need for microbial activity (particularly for their hydrolysing capabilities) in plant-material degradation, leading to the current bipartite view of the nutritional biology of the fungus-growing termites .
However, bacteria producing cellulolytic enzymes have been isolated from Odontotermes spp. as early as in the 50’s . Reports on the isolation of cellulose- and xylose-decomposing bacteria from hind guts of fungus-growing termites, mainly from Odontotermes and Macrotermes, have been published since [71,72,73]. Earlier work on the gut-bacteria composition of Macrotermitinae have provided the first evidence that the microbiotas resemble those of other termites and that fermentation products are accumulated [72,74,75]. More recently, the diversity of gut bacteria in fungus-growing termites has been studied, using culture-independent techniques. Bacterial clones (from the Cytophaga-Flexibacter-Bacteriodes phylum) known to be specialized in the degradation of plant fibres and proteins , likely constituents of the termite diet, were reported to constitute the major part of the gut flora of O.formosanus , M. gilvus  and M. michaelseni  workers. Recently, lignocellulase-positive bacterial clones from M. annandalei have been isolated , directly demonstrating that the gut bacteria of fungus-growing termites contribute with glycosidases to digestion. However, the relative contribution of gut bacteria in fungus-growing termites to degradation of plant material remains to be established.
Whenever a comparison has been made between microbial diversity in the termite gut and in the fungus comb, higher numbers and greater diversity of bacteria have been found in the termite gut (Macrotermes gilvus ; Odontotermes yunnanensis ). These findings, showing a consistent and unique termite gut flora, suggest co-evolution of the gut bacteria with the termite host. Although the bacterial community of the termite gut is commonly exchanged between colony members and likely transmitted to the next generation vertically via trophallaxis, promoting coevolutionary diversification along with the host, differences in diets (even within the same feeding group) inevitably influence the composition of the microbial community [78,82]. The few data available so far do not allow us to conclude if the termite gut microbiota is specific to certain taxa of termites, feeding groups or a combination of both. However, at least some specificity exists, as all studies to date show that a single termite species harbors several hundred species of gut bacteria unique to termites. The increasing interest in the gut-bacterial community of fungus-growing termites as a source of lignocellulase genes [80,83,84] will likely stimulate research towards a better understanding of the functional details of coevolution and the role of the gut bacteria therein.
7. Selection of Specific Fungal Strains When Starting a Colony
Whereas the termite gut-microbiota can be assumed to be mainly vertically inherited, the fungal symbiont is transmitted horizontally in most species (reviewed in ). We have discussed that transmission mode is not a good predictor of interaction specificity and argued that the specific role of the fungal symbiont may instead be an important factor to explain the observed differences in interaction specificity between species. But how is the right fungal symbiont selected and maintained, when a new colony starts without a fungal symbiont?
All studies analyzing the diversity of Termitomyces within colonies have shown that individual colonies of fungus-growing termites are associated with a single strain of Termitomyces, irrespective of symbiont transmission mode [13,30,33,34]. In all genera studied so far, the termite workers harvest the fungal nodules containing asexual spores [85,86,87]. These spores are mixed with foraged plant material in the gut of workers and deposited on the fungus comb, allowing rapid growth of a new mycelium and of new nodules, which are then consumed again. This within-colony propagation mode is universal for the entire clade of fungus-growing termites, although the details of division of labor between workers differ between genera [24,88]. During this continuous asexual propagation within a colony, the fungus is thus reared as a monoculture.
With horizontally transmitted symbionts, the first fungus garden will usually be established from sexual spores collected from the environment together with foraged plant material. Under these circumstances there will be ample opportunity that multiple spores are brought in to the colony. Experiments have shown that a monoculture emerges due to positive frequency dependent selection, genetic drift and selection for rapid asexual spore formation [24,30]. Subsequent colonization by other strains, which will initially be in a minority, will thus be prevented. This results in a life-time commitment between a termite society and a single fungal clone, so that the costs of acquiring a non-optimal symbiont at the start of a colony will be high.
These experiments thus clearly show how starting from a mixture of spores a single monoculture emerges. However, these experiments were performed with a mixture of symbionts, which all were isolated from a single species, and thus all were of a certain specific genotype. A different question is how, from a mixture of potentially good and potentially unsuited genotypes, a specific fungus is selected. The foraged material, including Termitomyces basidiospores, is ingested by the termite foragers, brought into the nest and deposited as feces to form the new fungus comb. In this first gut passage, it is known that the foraged plant substrate is mixed with the spores but, as argued earlier in this paper, there is also ample potential to further pre-treat this substrate by mechanical and enzymatic action through gut-derived enzymes, either from termite or bacterial origin. Because this masticated plant-material contains the fungal inoculum and is going to be the growth substrate of the fungal symbiont, the host has the opportunity to screen its symbionts on this substrate. According to this novel hypothesis, the pre-treatment of substrate thus determines the fungal symbiont and accounts for the observed partner specificity. The differences in the “selection” media offered to the symbiotic fungus may depend on differences in host-derived enzymes, but also in host diet and in the gut microbial community.
In this paper we have proposed the ruminant hypothesis. According to this novel hypothesis fungal enzymes and spores produced in nodules are mixed with the foraged plant material and enzymes of termite and bacterial origin. This mixture initially benefits the growth of the fungus, and facilitates efficient degradation of plant material, ultimately also to the benefit of the termites in the form of a nitrogen enriched food source. Furthermore, the mutualistic diversification observed in the fungus-growing termites suggests that the evolution and maintenance of specific host-fungal symbiont interactions is determined by the demands of the termites on their fungal symbiont to fulfill their nutritional requirements. The exact role of the termite gut bacteria remains to be studied in more detail. The isolation of ligno-cellulolytic bacteria indicates that this question deserves more attention. The extent to which bacterial metabolism is critical for the degradation of the plant material still remains an open question, as well as the extent to which the bacteria are mediating host-fungal association at the start of a new colony. We have argued that the termite host has the opportunity to select specific fungal symbionts by testing spores on the offered growth substrate, which consists of masticated plant material that has undergone a first gut passage and is thus potentially subject to bacterial and termite derived enzymes. We have argued that we need a more balanced view on the mutualism of fungus-growing termites, moving away from its currently bipartite and insect-focused perspective, to include the possible relevance of bacterial symbionts and to emphasize the perspective of the fungus, in order to fully understand coevolution, specificity patterns and the long-time stability of this fascinating mutualism.
D.K.A. was supported by The Netherlands Organization for Scientific Research (VIDI). The authors thank an anonymous reviewer for the constructive comments on an earlier version of this manuscript.
- Watson, R.A.; Pollack, J.B. How Symbiosis Can Guide Evolutio. In Proceedings of the 5th European Conference on Advances in Artificial Life (ECAL ’99), Lausanne, Switzerland, 15 September 1999; pp. 29–38.
- Margulis, L. Biodiversity: Molecular biological domains, symbiosis and kingdom origins. Biosystems 1992, 27, 39–51. [Google Scholar]
- Humphreys, C.P.; Franks, P.J.; Rees, M.; Bidartondo, M.I.; Leake, J.R.; Beerling, D.J. Mutualistic mycorrhiza-like symbiosis in the most ancient group of land plants. Nat. Commun. 2010, 1, 103. [Google Scholar]
- Groombridge, B. Global Biodiversity: Status of the Earth’s Living resources; Springer: Berlin, Heidelberg, Germany, 1992; Volume 1992. [Google Scholar]
- Erwin, T. The Biodiversity Question: How Many Species of Terrestrial Arthropods Are There? In Forest Canopies; Lowman, M.D., Rinker, H., Eds.; Elsevier: Amsterdam, The Netherlands, 2004; pp. 259–269. [Google Scholar]
- Gullan, P.; Cranston, P. The Insects: An Outline of Entomology, 4th ed.; Wiley-Blackwell: Hoboken, NJ, USA, 2010. [Google Scholar]
- Baumann, P. Biology of Bacteriocyte-Associated Endosymbionts of Plant Sap-Sucking Insects. In Annual Review of Microbiology; Annual Reviews: Palo Alto, CA, USA, 2005; Volume 59, pp. 155–189. [Google Scholar]
- Feldhaar, H.; Gross, R. Insects as hosts for mutualistic bacteria. Int. J. Med. Microbiol. 2009, 299, 1–8. [Google Scholar]
- Douglas, A.E. Nutritional interactions in insect-microbial symbioses: Aphids and their symbiotic bacteria Buchnera. Annu. Rev. Entomol. 1998, 43, 17–37. [Google Scholar]
- Shigenobu, S.; Wilson, A. Genomic revelations of a mutualism: The pea aphid and its obligate bacterial symbiont. Cell. Mol. Life Sci. 2011, 68, 1297–1309. [Google Scholar]
- Gibson, C.M.; Hunter, M.S. Extraordinarily widespread and fantastically complex: Comparative biology of endosymbiotic bacterial and fungal mutualists of insects. Ecol. Lett. 2010, 13, 223–234. [Google Scholar]
- Scott, J.J.; Oh, D.-C.; Yuceer, M.C.; Klepzig, K.D.; Clardy, J.; Currie, C.R. Bacterial protection of beetle-fungus mutualism. Science 2008, 322. [Google Scholar] [CrossRef]
- Aanen, D.K.; Eggleton, P.; Rouland-Lefevre, C.; Guldberg-Froslev, T.; Rosendahl, S.; Boomsma, J.J. the evolution of fungus-growing termites and their mutualistic fungal symbionts. Proc. Natl. Acad. Sci. USA 2002, 99, 14887–14892. [Google Scholar]
- Mueller, U.G.; Schultz, T.R.; Cameron, R.C.; Adams, R.M.M.; Malloch, D. The origin of the attine ant-fungus mutualism. Q. Rev. Biol. 2001, 76, 169–197. [Google Scholar]
- Farrell, B.D.; Sequeira, A.S.; O’Meara, B.C.; Normark, B.B.; Chung, J.H.; Jordal, B.H. The evolution of agriculture in beetles (Curculionidae: Scolytinae and Platypodinae). Evolution 2009, 55, 2011–2027. [Google Scholar]
- Aanen, D.K.; Eggleton, P. Fungus-growing termites originated in African rain forest. Curr. Biol. 2005, 15, 851–855. [Google Scholar]
- Nobre, T.; Koné, N.A.; Konaté, S.; Linsenmair, K.E.; Aanen, D.K. Dating the fungus-growing termites’ mutualism shows a mixture between ancient codiversification and recent symbiont dispersal across divergent hosts. Mol. Ecol. 2011, 20, 2619–2627. [Google Scholar]
- Jones, D.T.; Eggleton, P.; Bignell, D.E.; Roisin, Y.; Lo, N. Global Biogeography of Termites: A Compilation of Sources. In Biology of Termites: A Modern Synthesis; Springer: Amsterdam, The Netherlands, 2011; pp. 477–498. [Google Scholar]
- Wood, T.G.; Sands, W.A. The Role of Termites in Ecosystems. In Production Ecology of Ants and Termites; Brian, M.V., Ed.; Cambridge Univ. Press: Cambridge, UK, 1978; pp. 245–292. [Google Scholar]
- Buxton, R.D. Changes in the composition and activities of termite communities in relation to changing rainfall. Oecologia 1981, 51, 371–378. [Google Scholar]
- Wilson, E. The Insect Societies; Belknap Press of Harvard University Press: Cambridge, MA, USA, 1971. [Google Scholar]
- Darlington, J. Nutrition and Evolution in Fungus-Growing Termites. In Nourishment and Evolution in Insect Societies; Hunt, J.H., Nalepa, C.A., Eds.; Westview Press: Boulder, CO, USA, 1994; pp. 105–130. [Google Scholar]
- Leuthold, R.H.; Badertscher, S.; Imboden, H. The inoculation of newly formed fungus comb with Termitomyces in Macrotermes colonies (Isoptera, Macrotermitinae). Insectes Sociaux 1989, 36, 328–338. [Google Scholar] [CrossRef]
- Aanen, D.K. As you reap, so shall you sow: Coupling of inoculating and harvesting stabilizes the mutualism between termites and fungi. Biol. Lett. 2006, 2, 209–212. [Google Scholar]
- Korb, J.; Aanen, D.K. The evolution of uniparental transmission of fungal symbionts in fungus-growing termites (Macrotermitinae). Behav. Ecol. Sociobiol. 2003, 53, 65–71. [Google Scholar]
- Grassé, P.-P.; Noirot, C. La fondation de nouvelles sociétés par Bellicositermes natalensis Hav. Insectes Sociaux 1955, 2, 213–220. [Google Scholar] [CrossRef]
- Johnson, R. Colony development and establishment of the fungus comb in Microtermes sp. nr. usambaricus (Sjöstedt) (Isoptera: Macrotermitinae) from Nigeria. Insectes Sociaux 1981, 28, 3–12. [Google Scholar] [CrossRef]
- Johnson, R.A.; Thomas, R.J.; Wood, T.G.; Swift, M.J. The inoculation of the fungus comb in newly founded colonies of some species of the Macrotermitinae (Isoptera) from Nigeria. J. Nat. Hist. 1981, 15, 751–756. [Google Scholar]
- Nobre, T.; Fernandes, C.; Boomsma, J.J.; Korb, J.; Aanen, D.K. Farming termites determine the genetic population structure of Termitomyces fungal symbionts. Mol. Ecol. 2011, 20, 2023–2033. [Google Scholar] [CrossRef]
- Aanen, D.K.; de Fine Licht, H.H.; Debets, A.J.M.; Kerstes, N.A.G.; Hoekstra, R.F.; Boomsma, J.J. High symbiont relatedness stabilizes mutualistic cooperation in fungus-growing termites. Science 2009, 326, 1103–1106. [Google Scholar]
- Rouland-Lefevre, C. Symbiosis with Fungi. In Termites: Evolution, Sociality, Symbioses, Ecology; Abe, T., Bignell, D.E., Higashi, M., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2000; pp. 289–306. [Google Scholar]
- Rouland-Lefevre, C.; Diouf, M.N.; Brauman, A.; Neyra, M. Phylogenetic relationships in Termitomyces (family Agaricaceae) based on the nucleoticle sequence of ITS: A first approach to elucidate the evolutionary history of the symbiosis between fungus-growing termites and their fungi. Mol. Phylogenet. Evol. 2002, 22, 423–429. [Google Scholar]
- Aanen, D.K.; Ros, V.; de Fine Licht, H.; Mitchell, J.; de Beer, Z.W.; Slippers, B.; Rouland-LeFevre, C.; Boomsma, J. Patterns of interaction specificity of fungus-growing termites and Termitomyces symbionts in South Africa. BMC Evol. Biol. 2007, 7. [Google Scholar] [CrossRef]
- Katoh, H.; Miura, T.; Maekawa, K.; Shinzato, N.; Matsumoto, T. Genetic variation of symbiotic fungi cultivated by the macrotermitine termite Odontotermes formosanus (Isoptera: Termitidae) in the Ryukyu Archipelago. Mol. Ecol. 2002, 11, 1565–1572. [Google Scholar] [CrossRef]
- Nobre, T.; Eggleton, P.; Aanen, D.K. Vertical transmission as the key to the colonization of Madagascar by fungus-growing termites? Proc. R. Soc. B Biol. Sci. 2010, 277, 359–365. [Google Scholar] [CrossRef]
- De Fine Licht, H.H.; Boomsma, J.J.; Aanen, D.K. Presumptive horizontal symbiont transmission in the fungus-growing termite Macrotermes natalensis. Mol. Ecol. 2006, 15, 3131–3138. [Google Scholar] [CrossRef]
- Sun, Y.; Cheng, J. Hydrolysis of lignocellulosic materials for ethanol production: A review. Bioresour. Technol. 2002, 83, 1–11. [Google Scholar]
- Breznak, J.A.; Brune, A. Role of microorganisms in the digestion of lignocellulose by termites. Annu. Rev. Entomol. 1994, 39, 453–487. [Google Scholar]
- Bignell, D.E. Introduction to Symbiosi. In Termites: Evolution, Sociality, Symbioses, Ecology; Abe, T., Bignell, D.E., Higashi, M., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2000; pp. 189–208. [Google Scholar]
- Rouland-Lefèvre, C.; Bignell, D. Cultivation of Symbiotic Fungi by Termites of the Subfamily Macrotermitinae. In Symbiosis; volume 4, Seckbach, J., Ed.; Springer: Amsterdam, The Netherlands, 2004; pp. 731–756. [Google Scholar]
- Veivers, P.C.; Mühlemann, R.; Slaytor, M.; Leuthold, R.H.; Bignell, D.E. Digestion, diet and polyethism in two fungus-growing termites: Macrotermes subhyalinus Rambur and M. michaelseni Sjøstedt. J. Insect Physiol. 1991, 37, 675–682. [Google Scholar] [CrossRef]
- Wood, T.G.; Thomas, R.J. The Mutualistic Association between Macrotermitinae and Termitomyces. In Insect—Fungus Interactions; Wilding, N., Collins, N.M., Hammond, P.M., Webber, J.F., Eds.; Academic Press: London, UK, 1989; pp. 113–128. [Google Scholar]
- Grassé, P.-P.; Noirot, C. Le meule des termites champignonnistes et sa signification symbiotique. Ann. Sci. Nat. Zool. Biol. Anim. 1958, 11, 113–128. [Google Scholar]
- Martin, M.M.; Martin, J.S. Cellulose digestion in the midgut of the fungus-growing termite Macrotermes natalensis: The role of acquired digestive enzymes. Science 1978, 199, 1453–1455. [Google Scholar]
- Rouland-Lefevre, C.; Lenoir, F.; Lepage, M. The role of the symbiotic fungus in the digestive metabolism of several species of fungus-growing termites. Comp. Biochem. Physiol. 1991, 99A, 657–663. [Google Scholar]
- Bignell, D.E.; Eggleton, P. Termites in Ecosystems. In Termites: Evolution, Sociality, Symbioses, Ecology; Abe, T., Bignell, D.E., Higashi, M., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2000; pp. 363–387. [Google Scholar]
- Hyodo, F.; Tayasu, I.; Inoue, T.; Azuma, J.I.; Kudo, T.; Abe, T. Differential role of symbiotic fungi in lignin degradation and food provision for fungus-growing termites (Macrotermitinae: Isoptera). Funct. Ecol. 2003, 17, 186–193. [Google Scholar]
- Hyodo, F.; Inoue, T.; Azuma, J.I.; Tayasu, I.; Abe, T. Role of the mutualistic fungus in lignin degradation in the fungus-growing termite Macrotermes gilvus (Isoptera; Macrotermitinae). Soil Biol. Biochem. 2000, 32, 653–658. [Google Scholar] [CrossRef]
- Rohrmann, G. The origin, structure, and nutritional importance of the comb in two species of Macrotermitinae. Pedobiologia 1978, 18, 89–98. [Google Scholar]
- Abo-Khatwa, N. Cellulase of fungus-growing termites: A new hypothesis on its origin. Cell. Mol. Life Sci. 1978, 34, 559–560. [Google Scholar]
- Rouland, C.; Civas, A.; Renoux, J.; Petek, F. Purification and properties of cellulases from the termite Macrotermes mulleri (Termitidae, Macrotermitinae) and its symbiotic fungus Termitomyces sp. Comp. Biochem. Physiol. Part B Biochem. 1988, 91, 449–458. [Google Scholar] [CrossRef]
- Ikhouane, A.; Rouland-Lefevre, C. In Etude de la biodégradation, in vivo et in vitro, de polymères glucidiques par trois champignons du genre Termitomyces. Actes Colloques Insectes Sociaux 1996, 10, 101–109. [Google Scholar]
- Matoub, M.; Rouland, C. Purification and properties of the xylanases from the termite Macrotermes bellicosus and its symbiotic fungus Termitomyces sp. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 1995, 112, 629–635. [Google Scholar] [CrossRef]
- Faulet, B.M.; Niamke, S.; Gonnety, J.T.; Kouame, L.P. Purification and biochemical properties of a new thermostable xylanase from symbiotic fungus, Termitomyces sp. Afr. J. Biotechnol. 2006, 5, 273–282. [Google Scholar]
- Deng, T.F.; Chen, C.R.; Cheng, M.L.; Pan, C.Y.; Zhou, Y.; Mo, J.C. Differences in cellulase activity among different castes of Odontotermes formosanus (Isoptera : Termitidae) and the symbiotic fungus Termitomyces albuminosus. Sociobiology 2008, 51, 697–704. [Google Scholar]
- Yang, T.; Mo, J.C.; Cheng, J. Purification and some properties of cellulase from Odontotermes formosanus (Isoptera: Termitidae). Entomol. Sin. 2004, 11, 1–10. [Google Scholar]
- Sengupta, S.; Ghosh, A.K.; Sengupta, S. Purification and characterisation of a β-glucosidase (cellobiase) from a mushroom Termitomyces clypeatus. Biochim. Biophys. Acta (BBA) Protein Struct. Mol. Enzymol. 1991, 1076, 215–220. [Google Scholar] [CrossRef]
- Ghosh, A.K.; Banerjee, P.C.; Sengupta, S. Purification and properties of xylan hydrolase from mushroom Termitomyces clypeatus. Biochim. Biophys. Acta (BBA) Enzymol. 1980, 612, 143–152. [Google Scholar]
- Sinha, N.; Sengupta, S. Simultaneous production of α-arabinofuranosidase and xylanase by Termitomyces clypeatus. World J. Microbiol. Biotechnol. 1995, 11, 359–360. [Google Scholar] [CrossRef]
- Bose, S.; Mazumder, S.; Mukherjee, M. Laccase production by the white-rot fungus Termitomyces clypeatus. J. Basic Microbiol. 2007, 47, 127–131. [Google Scholar] [CrossRef]
- Bignell, D.E.; Slaytor, M.; Veivers, P.C.; Muhlemann, R.; Leuthold, R.H. Functions of symbiotic fungus gardens in higher termites of the genus Macrotermes: Evidence against the acquired enzyme hypothesis. Acta Microbiol. Immunol. Hung. 1994, 41, 391–401. [Google Scholar]
- Slaytor, M. Cellulose digestion in termites and cockroaches: What role do symbionts play? Comp. Biochem. Physiol. Part B Biochem. Mol. Biol. 1992, 103, 775–784. [Google Scholar]
- Badertscher, S.; Gerber, C.; Leuthold, R.H. Polyethism in food supply and processing in termite colonies of Macrotermes subhyalinus (Isoptera). Behav. Ecol. Sociobiol. 1983, 12, 115–119. [Google Scholar] [CrossRef]
- Traniello, J.F.A.; Leuthold, R.H. The Behavior and Ecology of Foraging in Termites. In Termites: Evolution, Sociality, Symbioses, Ecology; Abe, T., Bignell, D.E., Higashi, M., Eds.; Kluwer Academic Publishers: Dordrecht, The Netherlands, 2000; pp. 141–168. [Google Scholar]
- Swift, M.; Heal, O.; Anderson, M. Decomposition in Terrestrial Ecosystems; Blackwell Scientific: Oxford, UK, 1979; p. 372. [Google Scholar]
- Collins, N. The Utilization of Nitrogen Resources by Termites (Isoptera). In Nitrogen as an Ecological Factor; Lee, J., McNeill, S., Rorison, I., Eds.; Blackwell Scientific Publications: Oxford, UK, 1983; pp. 381–412. [Google Scholar]
- Martin, M.M. Acquired Enzymes in the Fungus-Growing Termite Macrotermes natalensis. In Invertebrate Microbial Interactions. Ingested Fungal Enzymes in Arthropod Biology; Comstock Publishing Associates: Ithaca, NY, USA, 1987; pp. 17–36. [Google Scholar]
- Martin, M.M.; Martin, J.S. The distribution and origins of the cellulolytic enzymes of the higher termite, Macrotermes natalensis. Physiol. Zool. 1979, 52, 11–21. [Google Scholar]
- Bignell, D.E. Morphology, Physiology, Biochemistry and Functional Design of the Termite Gut: An Evolutionary Wonderland. In Biology of Termites: A Modern Synthesis; Bignell, D.E., Roisin, Y., Lo, N., Eds.; Springer: Amsterdam, The Netherlands, 2011; pp. 375–412. [Google Scholar]
- Misra, J.; Ranganathan, V. Digestion of cellulose by the mound building termite, Termes (Cyclotermes) obesus (Rambur). Proc. Plant Sci. 1954, 39, 100–113. [Google Scholar]
- Pasti, M.B.; Belli, M.L. Cellulolytic activity of actinomycetes isolated from termites (Termitidae) gut. FEMS Microbiol. Lett. 1985, 26, 107–112. [Google Scholar]
- Paul, J.; Saxena, S.; Varma, A. Ultrastructural studies of the termite (Odontotermes obesus) gut microflora and its cellulolytic properties. World J. Microbiol. Biotechnol. 1993, 9, 108–112. [Google Scholar] [CrossRef]
- Varm, A.; Bala Krishna, K.; Jaishree, P.; Shailendra, S.; Helmut, K. Lignocellulose degradation by microorganisms from termite hills and termite guts: A survey on the present state of art. FEMS Microbiol. Rev. 1994, 15, 9–28. [Google Scholar]
- Yara, K.; Jahan, K.; Hayashi, H. In situ morphology of the gut microbiota of the fungus-growing termite Odontotermes formosanus (Termitidae: Macrotermitidae). Sociobiology 1989, 15, 2247–2260. [Google Scholar]
- Anklinmuhlemann, R.; Bignell, D.E.; Veivers, P.C.; Leuthold, R.H.; Slaytor, M. Morphological, microbiological and biochemical studies of the gut flora in the fungus-growing termite Macrotermes subhyalinus. J. Insect Physiol. 1995, 41, 929–940. [Google Scholar] [CrossRef]
- Shah, H.N.; Gharbia, S.E. Ecophysiology and taxonomy of bacteroides and related taxa. Clin. Infect. Dis. 1993, 16, S160–S167. [Google Scholar]
- Ohkuma, M.; Noda, S.; Hongoh, Y.; Kudo, T. Diverse bacteria related to the bacteroides subgroup of the CFB phylum within the gut symbiotic communities of various termites. Biosci. Biotechnol. Biochem. 2002, 66, 78–84. [Google Scholar]
- Hongoh, Y.; Ekpornprasit, L.; Inoue, T.; Moriya, S.; Trakulnaleamsai, S.; Ohkuma, M.; Noparatnaraporn, N.; Kudo, T. Intracolony variation of bacterial gut microbiota among castes and ages in the fungus-growing termite Macrotermes gilvus. Mol. Ecol. 2006, 15, 505–516. [Google Scholar]
- Mackenzie, L.M.; Muigai, A.T.; Osir, E.O.; Lwande, W.; Keller, M.; Toledo, G.; Boga, H.I. Bacterial diversity in the intestinal tract of the fungus-cultivating termite Macrotermes michaelseni (Sjöstedt). Afr. J. Biotechnol. 2007, 6. [Google Scholar]
- Liu, N.; Yan, X.; Zhang, M.; Xie, L.; Wang, Q.; Huang, Y.; Zhou, X.; Wang, S.; Zhou, Z. Microbiome of fungus-growing termites: A new reservoir for lignocellulase genes. Appl. Environ. Microbiol. 2011, 77, 48–56. [Google Scholar]
- Long, Y.-H.; Xie, L.; Liu, N.; Yan, X.; Li, M.-H.; Fan, M.-Z.; Wang, Q. Comparison of gut-associated and nest-associated microbial communities of a fungus-growing termite Odontotermes yunnanensis. Insect Sci. 2010, 17, 265–276. [Google Scholar] [CrossRef]
- Brauman, A.; Dore, J.; Eggleton, P.; Bignell, D.; Breznak, J.A.; Kane, M.D. Molecular phylogenetic profiling of prokaryotic communities in guts of termites with different feeding habits. FEMS Microbiol. Ecol. 2001, 35, 27–36. [Google Scholar]
- Ohkuma, M. Termite symbiotic systems: Efficient bio-recycling of lignocellulose. Appl. Microbiol. Biotechnol. 2003, 61, 1–9. [Google Scholar]
- Scharf, M.E.; Boucias, D.G. Potential of termite-based biomass pre-treatment strategies for use in bioethanol production. Insect Sci. 2010, 17, 166–174. [Google Scholar]
- Bathellier, J. Contribution à l’ etude systématique et biologique de termites de l’Indo-Chine. Faune Colonies Franc. 1927, 1, 125–365. [Google Scholar]
- De Fine Licht, H.H.; Andersen, A.; Aanen, D.K. Termitomyces sp associated with the termite Macrotermes natalensis has a heterothallic mating system and multinucleate cells. Mycol. Res. 2005, 109, 314–318. [Google Scholar] [CrossRef]
- Heim, R. Termites et Champignons; Société nouvelle de Éditions Boubée: Paris, France, 1977. [Google Scholar]
- Thomas, R.J. Distribution of Termitomyces Heim and other fungi in the nests and major workers of several nigerian Macrotermitinae. Soil Biol. Biochem. 1987, 19, 335–341. [Google Scholar] [CrossRef]
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