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Diagnostics 2014, 4(4), 165-180; doi:10.3390/diagnostics4040165

Screen Printed Carbon Electrode Based Electrochemical Immunosensor for the Detection of Dengue NS1 Antigen

Department of Medical Microbiology and Parasitology, School of Medical Science, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
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Author to whom correspondence should be addressed.
Received: 3 June 2014 / Revised: 11 September 2014 / Accepted: 20 October 2014 / Published: 20 November 2014
(This article belongs to the Special Issue In Vitro Diagnostics)
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Abstract

An electrochemical immunosensor modified with the streptavidin/biotin system on screen printed carbon electrodes (SPCEs) for the detection of the dengue NS1 antigen was developed in this study. Monoclonal anti-NS1 capture antibody was immobilized on streptavidin-modified SPCEs to increase the sensitivity of the assay. Subsequently, a direct sandwich enzyme linked immunosorbent assay (ELISA) format was developed and optimized. An anti-NS1 detection antibody conjugated with horseradish peroxidase enzyme (HRP) and 3,3,5,5'-tetramethybezidine dihydrochloride (TMB/H2O2) was used as an enzyme mediator. Electrochemical detection was conducted using the chronoamperometric technique, and electrochemical responses were generated at −200 mV reduction potential. The calibration curve of the immunosensor showed a linear response between 0.5 µg/mL and 2 µg/mL and a detection limit of 0.03 µg/mL. Incorporation of a streptavidin/biotin system resulted in a well-oriented antibody immobilization of the capture antibody and consequently enhanced the sensitivity of the assay. In conclusion, this immunosensor is a promising technology for the rapid and convenient detection of acute dengue infection in real serum samples. View Full-Text
Keywords: electrochemical immunosensor; dengue diagnosis; NS1; screen printed carbon electrode; streptavidin/biotin electrochemical immunosensor; dengue diagnosis; NS1; screen printed carbon electrode; streptavidin/biotin
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MDPI and ACS Style

Parkash, O.; Yean, C.Y.; Shueb, R.H. Screen Printed Carbon Electrode Based Electrochemical Immunosensor for the Detection of Dengue NS1 Antigen. Diagnostics 2014, 4, 165-180.

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