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Life 2016, 6(2), 22; doi:10.3390/life6020022

Effects of Low-Temperature Plasma-Sterilization on Mars Analog Soil Samples Mixed with Deinococcus radiodurans

1
Astrobiology Research Group, Center for Astronomy and Astrophysics, Technical University Berlin (TUB), Berlin 10623, Germany
2
Biomedical Applications of Plasma Technology, Institute for Electrical Engineering and Plasma Technology (AEPT), Ruhr University Bochum (RUB), Bochum 44801, Germany
3
School of the Environment, Washington State University, Pullman, WA 99164, USA
*
Author to whom correspondence should be addressed.
Academic Editor: David Deamer
Received: 1 March 2016 / Revised: 27 April 2016 / Accepted: 23 May 2016 / Published: 26 May 2016
(This article belongs to the Section Life Sciences)
View Full-Text   |   Download PDF [836 KB, uploaded 26 May 2016]   |  

Abstract

We used Ar plasma-sterilization at a temperature below 80 °C to examine its effects on the viability of microorganisms when intermixed with tested soil. Due to a relatively low temperature, this method is not thought to affect the properties of a soil, particularly its organic component, to a significant degree. The method has previously been shown to work well on spacecraft parts. The selected microorganism for this test was Deinococcus radiodurans R1, which is known for its remarkable resistance to radiation effects. Our results showed a reduction in microbial counts after applying a low temperature plasma, but not to a degree suitable for a sterilization of the soil. Even an increase of the treatment duration from 1.5 to 45 min did not achieve satisfying results, but only resulted in in a mean cell reduction rate of 75% compared to the untreated control samples. View Full-Text
Keywords: plasma sterilization; Deinococcus radiodurans; Mars; regolith plasma sterilization; Deinococcus radiodurans; Mars; regolith
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Schirmack, J.; Fiebrandt, M.; Stapelmann, K.; Schulze-Makuch, D. Effects of Low-Temperature Plasma-Sterilization on Mars Analog Soil Samples Mixed with Deinococcus radiodurans. Life 2016, 6, 22.

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