The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2
Abstract
:1. Introduction
2. Experimental Section
2.1. Strains and Culture Conditions
2.2. Determination of Glycogen Content
2.3. Electron Microscopy
2.4. Measurement of Respiratory Activity
2.5. Metabolome Analysis
2.6. RNA Gel Blot Analysis
2.7. Immunoblot Analysis
3. Results
3.1. Decrease of Glycogen Content during Dark Incubation
3.2. Changes in Respiratory Activity of the WT and the ∆cyabrB2 Mutant during Dark Incubation
3.3. Changes in Transcript Levels in the WT and ∆cyabrB2 Mutant Cells under Dark Conditions
3.4. Metabolome Analysis of the WT and ∆cyabrB2 Mutant Cells under Dark Conditions
3.5. Light-Dependent Changes in Transcript and Protein Amounts of cyAbrBs
3.6. Growth Property and Glycogen Content of the WT and ∆cyabrB2 Mutant Cells during Diurnal Light-Dark Cycles
4. Discussion
4.1. Physiological Roles of cyAbrB2 under Dark Conditions
4.2. Regulatory Mechanisms Working under Dark Conditions
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Hanai, M.; Sato, Y.; Miyagi, A.; Kawai-Yamada, M.; Tanaka, K.; Kaneko, Y.; Nishiyama, Y.; Hihara, Y. The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2 . Life 2014, 4, 770-787. https://doi.org/10.3390/life4040770
Hanai M, Sato Y, Miyagi A, Kawai-Yamada M, Tanaka K, Kaneko Y, Nishiyama Y, Hihara Y. The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2 . Life. 2014; 4(4):770-787. https://doi.org/10.3390/life4040770
Chicago/Turabian StyleHanai, Masamitsu, Yusuke Sato, Atsuko Miyagi, Maki Kawai-Yamada, Kyoko Tanaka, Yasuko Kaneko, Yoshitaka Nishiyama, and Yukako Hihara. 2014. "The Effects of Dark Incubation on Cellular Metabolism of the Wild Type Cyanobacterium Synechocystis sp. PCC 6803 and a Mutant Lacking the Transcriptional Regulator cyAbrB2 " Life 4, no. 4: 770-787. https://doi.org/10.3390/life4040770