Next Article in Journal
Psycho-Social Issues in Mine Emergencies: The Impact on the Individual, the Organization and the Community
Next Article in Special Issue
Cyanobacteria as Biocatalysts for Carbonate Mineralization
Previous Article in Journal
Dehydration and Rehydration of Carbonated Fluor- and Hydroxylapatite
Previous Article in Special Issue
Biochemical Change at the Setting-up of the Crossed-Lamellar Layer in Nerita undata Shell (Mollusca, Gastropoda)
Article Menu

Export Article

Open AccessArticle
Minerals 2012, 2(2), 118-128; doi:10.3390/min2020118

A Novel Method for Culturing of Leptothrix sp. Strain OUMS1 in Natural Conditions

1
Department of Material Chemistry, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan
2
Laboratory of Plant Pathology, Graduate School of Environmental Science, Okayama University, Okayama 700-8530, Japan
*
Author to whom correspondence should be addressed.
Received: 8 February 2012 / Revised: 23 March 2012 / Accepted: 15 May 2012 / Published: 23 May 2012
(This article belongs to the Special Issue Advances in Biominerals)
View Full-Text   |   Download PDF [419 KB, uploaded 23 May 2012]   |  

Abstract

Although some strains of Leptothrix spp. isolated from aquatic environments have been characterized by culturing them in laboratory conditions, they often show morphological and chemical features distinct from those found in natural environments. To resolve this discrepancy, a novel cultivation method was devised for culturing such strains in natural groundwater. Leptothrix sp. strain OUMS1 was pre-cultured in a medium lacking Fe for 2 days, and then injected into a small dialysis tube bag and immersed in a container with continuously flowing groundwater for 1–3 and 14 days. Microscopic analysis of the initial phase of sheath formation and arbitrary comparisons with medium cultures revealed that in groundwater the surface coat of the sheath comprised much thinner fibrils, and an inner sheath wall that was much thinner and more indistinct compared with medium cultures. These differences were probably attributable to poorer secretion from the cell surface in groundwater conditions. A nutrient-rich medium likely activates cell metabolism and promotes secretion, resulting in a thicker inner sheath wall and thicker outer coat fibrils. Aqueous-phase Fe was deposited on immature sheaths in a similar manner in both cultures. These results indicate that laboratory culture of isolated microbes does not always reflect their characteristics in natural environments.
Keywords: Leptothrix sp. OUMS1; novel culturing method in natural groundwater; sheath formation; bacterial carbohydrates; bacterial exudation; deposition of aqueous-phase iron Leptothrix sp. OUMS1; novel culturing method in natural groundwater; sheath formation; bacterial carbohydrates; bacterial exudation; deposition of aqueous-phase iron
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Suzuki, T.; Ishihara, H.; Furutani, M.; Shiraishi, T.; Kunoh, H.; Takada, J. A Novel Method for Culturing of Leptothrix sp. Strain OUMS1 in Natural Conditions. Minerals 2012, 2, 118-128.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Minerals EISSN 2075-163X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top