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Antibodies 2013, 2(1), 1-8; doi:10.3390/antib2010001

Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1)

1,* , 1
1 Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, 520-2192, Japan 2 Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, 259-1193, Japan 3 Advanced Molecular Epidemiology Research Institute, Faculty of Medicine, Yamagata University, Yamagata, 990-9585, Japan
* Authors to whom correspondence should be addressed.
Received: 9 November 2012 / Revised: 10 December 2012 / Accepted: 14 December 2012 / Published: 21 December 2012
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TATA-box binding protein-associated factor 1 (TAF1), the largest subunit of the transcription factor IID complex, plays an important role in the RNA polymerase II-mediated gene transcription pathway regulating the transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1) may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. The present study reports the preparation and properties of a monoclonal antibody directed against N-TAF1. The monoclonal antibody, 3A-11F, specifically recognized N-TAF1 protein with no reactivity to TAF1 protein, as evidenced by immunocytochemistry and immunoprecipitation using cultured cells expressing recombinant N-TAF1 or TAF1 protein. Immunohistochemistry using 3A-11F showed that N-TAF1-imunoreactivity was detected in the nuclear region of neurons in the rat brain. The 3A-11F monoclonal antibody promises to be a useful tool for determining the expression pattern and biological function of N-TAF1 in the brain.
Keywords: TAF1; transcription factor; immunohistochemistry; immunoprecipitation TAF1; transcription factor; immunohistochemistry; immunoprecipitation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Makino, S.; Masuda, C.; Ando, S.; Tamiya, G.; Tooyama, I. Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1). Antibodies 2013, 2, 1-8.

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