• Submit to Antibodies Login Register
• MDPI
• Journals A-Z
• For Authors
• For Editors
• About
• Open Access Policy

• Abstract
• Full-Text PDF [355 KB]
• Full-Text XML
• Article Versions Notes

• Article Statistics
• Google Scholar
• Order Reprints

• Cite this article
• Export to BibTeX
• Export to EndNote

• [+] on DOAJ
• Uto, T
• Tung, NHuu
• Morinaga, O
• Shoyama, Y
• [+] on Google Scholar
• Uto, T
• Tung, NHuu
• Morinaga, O
• Shoyama, Y
• [+] on PubMed
• Uto, T
• Tung, NHuu
• Morinaga, O
• Shoyama, Y

•  CiteULike
•  Facebook
•  Mendeley
•  Twitter

This article is

• freely available
• re-usable

Antibodies 2012, 1(3), 294-307; doi:10.3390/antib1030294

Review

Preparation of Knockout Extract by Immunoaffinity Column and Its Application

Takuhiro Uto, Nguyen Huu Tung, Osamu Morinaga and Yukihiro Shoyama*

Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch-Cho, Sasebo, Nagasaki 859-3298, Japan

* Author to whom correspondence should be addressed.

Received: 28 August 2012; in revised form: 30 November 2012 / Accepted: 4 December 2012 / Published: 6 December 2012

(This article belongs to the Special Issue Monoclonal Antibody Preparation against Natural Product and Its Application)

Download PDF Full-Text [355 KB, uploaded 6 December 2012 12:09 CET]

Abstract: Importance of herbal medicines have recently increased owing to rising interest in their health benefits. However, medicinal plant extracts are complex mixtures of phytochemicals that act synergistically or additively on specific and/or multiple molecular and cellular targets. Thus, it is difficult to examine the actual pharmacological roles of active compounds in plant extracts. This review describes a new strategy for isolating target compounds from plant extracts using immunoaffinity columns coupled with monoclonal antibodies (mAbs) against natural compounds. Through one-step purification using mAb-coupled immunoaffinity columns, we succeeded in preparing a knockout (KO) extract, which contains all components except the target compound. Furthermore, we investigated the pharmacological effects of the KO extract to reveal the actual effects of a bioactive compound in the crude extract. This approach may help determine the potential function of target compounds in herbal medicines.

Keywords: monoclonal antibody; one-step purification; ginseng; ginsenosides; licorice; glycyrrhizin

Article Statistics

Cite This Article