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Genes 2018, 9(8), 399; https://doi.org/10.3390/genes9080399

OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis

1
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China
2
College of Life Science, Northeast Agricultural University, Harbin 150030, China
3
College of Mathematics and Information Sciences, Guangxi University, Nanning 530004, China
These authors have contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 28 June 2018 / Revised: 28 July 2018 / Accepted: 30 July 2018 / Published: 6 August 2018
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

Ovate Family Protein1 (OFP1) is a regulator, and it is suspected to be involved in plant growth and development. Meanwhile, Arabidopsis Thaliana Homeobox (ATH1), a BEL1-like homeodomain (HD) transcription factor, is known to be involved in regulating stem growth, flowering time and flower basal boundary development in Arabidopsis. Previous large-scale yeast two-hybrid studies suggest that ATH1 possibly interact with OFP1, but this interaction is yet unverified. In our study, the interaction of OFP1 with ATH1 was verified using a directional yeast two-hybrid system and bimolecular fluorescence complementation (BiFC). Our results also demonstrated that the OFP1-ATH1 interaction is mainly controlled by the HD domain of ATH1. Meanwhile, we found that ATH1 plays the role of transcriptional repressor to regulate plant development and that OFP1 can enhance ATH1 repression function. Regardless of the mechanism, a putative functional role of ATH1-OFP1 may be to regulate the expression of the both the GA20ox1 gene, which is involved in gibberellin (GA) biosynthesis and control of stem elongation, and the Flowering Locus C (FLC) gene, which inhibits transition to flowering. Ultimately, the regulatory functional mechanism of OFP1-ATH1 may be complicated and diverse according to our results, and this work lays groundwork for further understanding of a unique and important protein–protein interaction that influences flowering time, stem development, and flower basal boundary development in plants. View Full-Text
Keywords: OFP1; ATH1; interaction; stem elongation; flowering; boundary OFP1; ATH1; interaction; stem elongation; flowering; boundary
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Zhang, L.; Sun, L.; Zhang, X.; Zhang, S.; Xie, D.; Liang, C.; Huang, W.; Fan, L.; Fang, Y.; Chang, Y. OFP1 Interaction with ATH1 Regulates Stem Growth, Flowering Time and Flower Basal Boundary Formation in Arabidopsis. Genes 2018, 9, 399.

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