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Genes 2017, 8(4), 121; doi:10.3390/genes8040121

A Transcriptomic Comparison of Two Bambara Groundnut Landraces under Dehydration Stress

1
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Nottingham LE12 5RD, UK
2
Crops for the Future, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
3
Centre for Plant Integrative Biology, University of Nottingham, Sutton Bonington Campus, Nottingham LE12 5RD, UK
4
School of Animal Rural and Environmental Sciences, Nottingham Trent University, Clifton Campus, Nottingham NG11 8NS, UK
*
Author to whom correspondence should be addressed.
Academic Editor: Qingyi Yu
Received: 20 February 2017 / Revised: 6 April 2017 / Accepted: 10 April 2017 / Published: 18 April 2017
(This article belongs to the Section Plant Genetics and Genomics)
View Full-Text   |   Download PDF [1343 KB, uploaded 18 April 2017]   |  

Abstract

The ability to grow crops under low-water conditions is a significant advantage in relation to global food security. Bambara groundnut is an underutilised crop grown by subsistence farmers in Africa and is known to survive in regions of water deficit. This study focuses on the analysis of the transcriptomic changes in two bambara groundnut landraces in response to dehydration stress. A cross-species hybridisation approach based on the Soybean Affymetrix GeneChip array has been employed. The differential gene expression analysis of a water-limited treatment, however, showed that the two landraces responded with almost completely different sets of genes. Hence, both landraces with very similar genotypes (as assessed by the hybridisation of genomic DNA onto the Soybean Affymetrix GeneChip) showed contrasting transcriptional behaviour in response to dehydration stress. In addition, both genotypes showed a high expression of dehydration-associated genes, even under water-sufficient conditions. Several gene regulators were identified as potentially important. Some are already known, such as WRKY40, but others may also be considered, namely PRR7, ATAUX2-11, CONSTANS-like 1, MYB60, AGL-83, and a Zinc-finger protein. These data provide a basis for drought trait research in the bambara groundnut, which will facilitate functional genomics studies. An analysis of this dataset has identified that both genotypes appear to be in a dehydration-ready state, even in the absence of dehydration stress, and may have adapted in different ways to achieve drought resistance. This will help in understanding the mechanisms underlying the ability of crops to produce viable yields under drought conditions. In addition, cross-species hybridisation to the soybean microarray has been shown to be informative for investigating the bambara groundnut transcriptome. View Full-Text
Keywords: Bambara groundnut; landraces; dehydration stress; cross-species microarray analysis Bambara groundnut; landraces; dehydration stress; cross-species microarray analysis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Khan, F.; Chai, H.H.; Ajmera, I.; Hodgman, C.; Mayes, S.; Lu, C. A Transcriptomic Comparison of Two Bambara Groundnut Landraces under Dehydration Stress. Genes 2017, 8, 121.

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