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Genes 2017, 8(4), 109; doi:10.3390/genes8040109

Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars

Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
Department of Biology, Chungbuk National University, Cheongju 28644, Korea
Department of Forest Genetic Resources, National Institute of Forest Science, Suwon 16631, Korea
Theragen Etex Bio Institute, Suwon 16229, Korea
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 6 January 2017 / Revised: 28 February 2017 / Accepted: 21 March 2017 / Published: 24 March 2017
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [197 KB, uploaded 24 March 2017]


The shiitake mushroom (Lentinula edodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database of L. edodes. These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2–11; the observed and expected heterozygosity were 0.00–1.00 and 0.10–0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications. View Full-Text
Keywords: shiitake mushroom; simple sequence repeat (SSR) marker; whole genome sequencing shiitake mushroom; simple sequence repeat (SSR) marker; whole genome sequencing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Lee, H.-Y.; Moon, S.; Shim, D.; Hong, C.P.; Lee, Y.; Koo, C.-D.; Chung, J.-W.; Ryu, H. Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars. Genes 2017, 8, 109.

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