Next Article in Journal
Anatomy of Mammalian Replication Domains
Previous Article in Journal
Dystrophic Cardiomyopathy—Potential Role of Calcium in Pathogenesis, Treatment and Novel Therapies
Article Menu

Export Article

Open AccessCommunication
Genes 2017, 8(4), 109; doi:10.3390/genes8040109

Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars

1
Department of Forest Science, Chungbuk National University, Cheongju 28644, Korea
2
Department of Biology, Chungbuk National University, Cheongju 28644, Korea
3
Department of Forest Genetic Resources, National Institute of Forest Science, Suwon 16631, Korea
4
Theragen Etex Bio Institute, Suwon 16229, Korea
5
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Received: 6 January 2017 / Revised: 28 February 2017 / Accepted: 21 March 2017 / Published: 24 March 2017
(This article belongs to the Section Molecular Genetics)
View Full-Text   |   Download PDF [197 KB, uploaded 24 March 2017]

Abstract

The shiitake mushroom (Lentinula edodes) is one of the most popular edible mushrooms in the world and has attracted attention for its value in medicinal and pharmacological uses. With recent advanced research and techniques, the agricultural cultivation of the shiitake mushroom has been greatly increased, especially in East Asia. Additionally, demand for the development of new cultivars with good agricultural traits has been greatly enhanced, but the development processes are complicated and more challenging than for other edible mushrooms. In this study, we developed 44 novel polymorphic simple sequence repeat (SSR) markers for the determination of shiitake mushroom cultivars based on a whole genome sequencing database of L. edodes. These markers were found to be polymorphic and reliable when screened in 23 shiitake mushroom cultivars. For the 44 SSR markers developed in this study, the major allele frequency ranged from 0.13 to 0.94; the number of genotypes and number of alleles were each 2–11; the observed and expected heterozygosity were 0.00–1.00 and 0.10–0.90, respectively; and the polymorphic information content value ranged from 0.10 to 0.89. These new markers can be used for molecular breeding, the determination of cultivars, and other applications. View Full-Text
Keywords: shiitake mushroom; simple sequence repeat (SSR) marker; whole genome sequencing shiitake mushroom; simple sequence repeat (SSR) marker; whole genome sequencing
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Lee, H.-Y.; Moon, S.; Shim, D.; Hong, C.P.; Lee, Y.; Koo, C.-D.; Chung, J.-W.; Ryu, H. Development of 44 Novel Polymorphic SSR Markers for Determination of Shiitake Mushroom (Lentinula edodes) Cultivars. Genes 2017, 8, 109.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top