Next Article in Journal
Sequencing and De Novo Assembly of the Toxicodendron radicans (Poison Ivy) Transcriptome
Previous Article in Journal
Impact of Lateral Transfers on the Genomes of Lepidoptera
Article Menu

Export Article

Open AccessArticle
Genes 2017, 8(11), 314; doi:10.3390/genes8110314

A Microbiomic Analysis in African Americans with Colonic Lesions Reveals Streptococcus sp.VT162 as a Marker of Neoplastic Transformation

1
Department of Pathology and Department of Medicine, Microbiology and Cancer Center, College of Medicine, Howard University, 2041 Georgia Avenue, Washington, DC 20060, USA
2
J. Craig Venter Institute, La Jolla, CA 92037, USA
3
HiThru Analytics, Laurel, MD 20877, USA
4
MRDNA/Molecular Research LP, Shallowater, TX 79363, USA
5
J. Craig Venter Institute, Rockville, MD 20850, USA
6
Systems and Translational Sciences, RTI International, NC 27709, USA
7
Laboratory of Microbiology, Department of Agrotechnology and Food Sciences, Wageningen University, 6708 PB Wageningen, The Netherlands
8
Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, CUHK Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China
Present address: University of North Carolina at Chapel Hill, Common Fund Eastern Regional Comprehensive Metabolomics Resource Core, Chapel Hill, NC 27599, USA
*
Author to whom correspondence should be addressed.
Received: 30 August 2017 / Revised: 19 October 2017 / Accepted: 26 October 2017 / Published: 9 November 2017
(This article belongs to the Special Issue Intestinal Microbes and Cancer)
View Full-Text   |   Download PDF [7504 KB, uploaded 9 November 2017]   |  

Abstract

Increasing evidence suggests a role of the gut microbiota in colorectal carcinogenesis (CRC). To detect bacterial markers of colorectal cancer in African Americans a metabolomic analysis was performed on fecal water extracts. DNA from stool samples of adenoma and healthy subjects and from colon cancer and matched normal tissues was analyzed to determine the microbiota composition (using 16S rDNA) and genomic content (metagenomics). Metagenomic functions with discriminative power between healthy and neoplastic specimens were established. Quantitative Polymerase Chain Reaction (q-PCR) using primers and probes specific to Streptococcus sp. VT_162 were used to validate this bacterium association with neoplastic transformation in stool samples from two independent cohorts of African Americans and Chinese patients with colorectal lesions. The metabolomic analysis of adenomas revealed low amino acids content. The microbiota in both cancer vs. normal tissues and adenoma vs. normal stool samples were different at the 16S rRNA gene level. Cross-mapping of metagenomic data led to 9 markers with significant discriminative power between normal and diseased specimens. These markers identified with Streptococcus sp. VT_162. Q-PCR data showed a statistically significant presence of this bacterium in advanced adenoma and cancer samples in an independent cohort of CRC patients. We defined metagenomic functions from Streptococcus sp. VT_162 with discriminative power among cancers vs. matched normal and adenomas vs. healthy subjects’ stools. Streptococcus sp. VT_162 specific 16S rDNA was validated in an independent cohort. These findings might facilitate non-invasive screening for colorectal cancer. View Full-Text
Keywords: colorectal cancer; microbiota; African Americans; metabolomics; metagenomic linkage groups colorectal cancer; microbiota; African Americans; metabolomics; metagenomic linkage groups
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Brim, H.; Yooseph, S.; Lee, E.; Sherif, Z.; Abbas, M.; Laiyemo, A.O.; Varma, S.; Torralba, M.; Dowd, S.E.; Nelson, K.E.; Pathmasiri, W.; Sumner, S.; de Vos, W.; Liang, Q.; Yu, J.; Zoetendal, E.; Ashktorab, H. A Microbiomic Analysis in African Americans with Colonic Lesions Reveals Streptococcus sp.VT162 as a Marker of Neoplastic Transformation. Genes 2017, 8, 314.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top