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Genes 2017, 8(11), 317; doi:10.3390/genes8110317

Sequencing and De Novo Assembly of the Toxicodendron radicans (Poison Ivy) Transcriptome

1
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97330, USA
2
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA 24061, USA
*
Author to whom correspondence should be addressed.
Received: 31 July 2017 / Revised: 12 October 2017 / Accepted: 12 October 2017 / Published: 10 November 2017
(This article belongs to the Section Plant Genetics and Genomics)
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Abstract

Contact with poison ivy plants is widely dreaded because they produce a natural product called urushiol that is responsible for allergenic contact delayed-dermatitis symptoms lasting for weeks. For this reason, the catchphrase most associated with poison ivy is “leaves of three, let it be”, which serves the purpose of both identification and an appeal for avoidance. Ironically, despite this notoriety, there is a dearth of specific knowledge about nearly all other aspects of poison ivy physiology and ecology. As a means of gaining a more molecular-oriented understanding of poison ivy physiology and ecology, Next Generation DNA sequencing technology was used to develop poison ivy root and leaf RNA-seq transcriptome resources. De novo assembled transcriptomes were analyzed to generate a core set of high quality expressed transcripts present in poison ivy tissue. The predicted protein sequences were evaluated for similarity to SwissProt homologs and InterProScan domains, as well as assigned both GO terms and KEGG annotations. Over 23,000 simple sequence repeats were identified in the transcriptome, and corresponding oligo nucleotide primer pairs were designed. A pan-transcriptome analysis of existing Anacardiaceae transcriptomes revealed conserved and unique transcripts among these species. View Full-Text
Keywords: Toxicodendron radicans; poison ivy; urushiol; contact dermatitis; skin rash; transcriptome; Anacardiaceae Toxicodendron radicans; poison ivy; urushiol; contact dermatitis; skin rash; transcriptome; Anacardiaceae
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Weisberg, A.J.; Kim, G.; Westwood, J.H.; Jelesko, J.G. Sequencing and De Novo Assembly of the Toxicodendron radicans (Poison Ivy) Transcriptome. Genes 2017, 8, 317.

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