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Genes 2017, 8(10), 255; doi:10.3390/genes8100255

De Novo Assembly and Analysis of Tartary Buckwheat (Fagopyrum tataricum Garetn.) Transcriptome Discloses Key Regulators Involved in Salt-Stress Response

1,2,3,†,* , 1,2,3,†
,
3
,
1,2,3
,
1,2,3
,
1,2,3
,
1,2,3
and
1,2,3,*
1
Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture, Chengdu 610106, China
2
National Research and Development Center for Coarse Cereal Processing, Chengdu 610106, China
3
College of Pharmacy and Biological Engineering, Chengdu University, Chengdu 610106, China
These authors have contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 14 August 2017 / Revised: 15 September 2017 / Accepted: 15 September 2017 / Published: 3 October 2017
(This article belongs to the Special Issue Genetic Regulation of Abiotic Stress Responses)
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Abstract

Soil salinization has been a tremendous obstacle for agriculture production. The regulatory networks underlying salinity adaption in model plants have been extensively explored. However, limited understanding of the salt response mechanisms has hindered the planting and production in Fagopyrum tataricum, an economic and health-beneficial plant mainly distributing in southwest China. In this study, we performed physiological analysis and found that salt stress of 200 mM NaCl solution significantly affected the relative water content (RWC), electrolyte leakage (EL), malondialdehyde (MDA) content, peroxidase (POD) and superoxide dismutase (SOD) activities in tartary buckwheat seedlings. Further, we conducted transcriptome comparison between control and salt treatment to identify potential regulatory components involved in F. tataricum salt responses. A total of 53.15 million clean reads from control and salt-treated libraries were produced via an Illumina sequencing approach. Then we de novo assembled these reads into a transcriptome dataset containing 57,921 unigenes with N50 length of 1400 bp and total length of 44.5 Mb. A total of 36,688 unigenes could find matches in public databases. GO, KEGG and KOG classification suggested the enrichment of these unigenes in 56 sub-categories, 25 KOG, and 273 pathways, respectively. Comparison of the transcriptome expression patterns between control and salt treatment unveiled 455 differentially expressed genes (DEGs). Further, we found the genes encoding for protein kinases, phosphatases, heat shock proteins (HSPs), ATP-binding cassette (ABC) transporters, glutathione S-transferases (GSTs), abiotic-related transcription factors and circadian clock might be relevant to the salinity adaption of this species. Thus, this study offers an insight into salt tolerance mechanisms, and will serve as useful genetic information for tolerant elite breeding programs in future. View Full-Text
Keywords: Fagopyrum tataricum; salt stress; transcriptome; Illumina sequencing; expression analysis Fagopyrum tataricum; salt stress; transcriptome; Illumina sequencing; expression analysis
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MDPI and ACS Style

Wu, Q.; Bai, X.; Zhao, W.; Xiang, D.; Wan, Y.; Yan, J.; Zou, L.; Zhao, G. De Novo Assembly and Analysis of Tartary Buckwheat (Fagopyrum tataricum Garetn.) Transcriptome Discloses Key Regulators Involved in Salt-Stress Response. Genes 2017, 8, 255.

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