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Genes 2017, 8(1), 25; doi:10.3390/genes8010025

AAC as a Potential Target Gene to Control Verticillium dahliae

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
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Authors to whom correspondence should be addressed.
Academic Editors: Wenyi Gu and Paolo Cinelli
Received: 20 September 2016 / Revised: 28 December 2016 / Accepted: 5 January 2017 / Published: 10 January 2017
(This article belongs to the Special Issue RNA Interference 2016)
View Full-Text   |   Download PDF [6499 KB, uploaded 10 January 2017]   |  

Abstract

Verticillium dahliae invades the roots of host plants and causes vascular wilt, which seriously diminishes the yield of cotton and other important crops. The protein AAC (ADP, ATP carrier) is responsible for transferring ATP from the mitochondria into the cytoplasm. When V. dahliae protoplasts were transformed with short interfering RNAs (siRNAs) targeting the VdAAC gene, fungal growth and sporulation were significantly inhibited. To further confirm a role for VdAAC in fungal development, we generated knockout mutants (ΔVdACC). Compared with wild-type V. dahliae (Vd wt), ΔVdAAC was impaired in germination and virulence; these impairments were rescued in the complementary strains (ΔVdAAC-C). Moreover, when an RNAi construct of VdAAC under the control of the 35S promoter was used to transform Nicotiana benthamiana, the expression of VdAAC was downregulated in the transgenic seedlings, and they had elevated resistance against V. dahliae. The results of this study suggest that VdAAC contributes to fungal development, virulence and is a promising candidate gene to control V. dahliae. In addition, RNAi is a highly efficient way to silence fungal genes and provides a novel strategy to improve disease resistance in plants. View Full-Text
Keywords: Verticillium dahliae; VdAAC; RNAi; growth; virulence Verticillium dahliae; VdAAC; RNAi; growth; virulence
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Su, X.; Rehman, L.; Guo, H.; Li, X.; Zhang, R.; Cheng, H. AAC as a Potential Target Gene to Control Verticillium dahliae. Genes 2017, 8, 25.

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