Next Article in Journal
Replication-Associated Recombinational Repair: Lessons from Budding Yeast
Previous Article in Journal
Telomere Transcripts Target Telomerase in Human Cancer Cells
Article Menu

Export Article

Open AccessArticle
Genes 2016, 7(8), 47; doi:10.3390/genes7080047

Molecular Cytogenetics in Trough Shells (Mactridae, Bivalvia): Divergent GC-Rich Heterochromatin Content

Departamento de Bioquímica, Xenética e Inmunoloxía, Universidade de Vigo, E-36310 Vigo, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Montserrat Corominas
Received: 29 June 2016 / Revised: 3 August 2016 / Accepted: 8 August 2016 / Published: 16 August 2016
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
View Full-Text   |   Download PDF [4659 KB, uploaded 16 August 2016]   |  

Abstract

The family Mactridae is composed of a diverse group of marine organisms, commonly known as trough shells or surf clams, which illustrate a global distribution. Although this family includes some of the most fished and cultured bivalve species, their chromosomes are poorly studied. In this work, we analyzed the chromosomes of Spisula solida, Spisula subtruncata and Mactra stultorum by means of fluorochrome staining, C-banding and fluorescent in situ hybridization using 28S ribosomal DNA (rDNA), 5S rDNA, H3 histone gene and telomeric probes. All three trough shells presented 2n = 38 chromosomes but different karyotype compositions. As happens in most bivalves, GC-rich regions were limited to the nucleolus organizing regions in Spisula solida. In contrast, many GC-rich heterochromatic bands were detected in both Spisula subtruncata and Mactra stultorum. Although the three trough shells presented single 5S rDNA and H3 histone gene clusters, their chromosomal locations differed. Regarding major rDNA clusters, while Spisula subtruncata presented a single cluster, both Spisula solida and Mactra stultorum showed two. No evidence of intercalary telomeric signals was detected in these species. The molecular cytogenetic characterization of these taxa will contribute to understanding the role played by chromosome changes in the evolution of trough shells. View Full-Text
Keywords: trough shells; chromosome; heterochromatin; fluorescent in situ hybridization; histone genes; ribosomal RNA genes trough shells; chromosome; heterochromatin; fluorescent in situ hybridization; histone genes; ribosomal RNA genes
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

García-Souto, D.; Pérez-García, C.; Kendall, J.; Pasantes, J.J. Molecular Cytogenetics in Trough Shells (Mactridae, Bivalvia): Divergent GC-Rich Heterochromatin Content. Genes 2016, 7, 47.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top