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Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori
School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China
* Authors to whom correspondence should be addressed.
Received: 18 March 2013; in revised form: 7 May 2013 / Accepted: 15 May 2013 / Published: 30 May 2013
Abstract: The Scribble protein complex genes, consisting of lethal giant larvae (Lgl), discs large (Dlg) and scribble (Scrib) genes, are components of an evolutionarily conserved genetic pathway that links the cell polarity in cells of humans and Drosophila. The tissue expression and developmental changes of the Scribble protein complex genes were documented using qRT-RCR method. The Lgl and Scrib genes could be detected in all the experimental tissues, including fat body, midgut, testis/ovary, wingdisc, trachea, malpighian tubule, hemolymph, prothoracic gland and silk gland. The Dlg gene, mainly expressed only in testis/ovary, could not be detected in prothoracic gland and hemolymph. In fat body, there were two higher expression stages of the three genes. The highest peak of the expression of the Lgl and Scrib genes in wingdisc lay at the 1st day of the 5th instar, but the Dlg gene was at 3rd day of 5th instar. The above results indicate that Scribble complex genes are involved in the process of molting and development of the wingdisc in the silkworm. This will be useful in the future for the elucidation of the detailed biological function of the three genes Scrib, Dlg and Lgl in B. mori.
Keywords: lethal giant larvae; discs large; scribble; developmental expression; tissue expression; Bombyx mori
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Qi, H.-S.; Liu, S.-M.; Li, S.; Wei, Z.-J. Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori. Genes 2013, 4, 264-274.
Qi H-S, Liu S-M, Li S, Wei Z-J. Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori. Genes. 2013; 4(2):264-274.
Qi, Hai-Sheng; Liu, Shu-Min; Li, Sheng; Wei, Zhao-Jun. 2013. "Molecular Expression of the Scribble Complex Genes, Dlg, Scrib and Lgl, in Silkworm, Bombyx mori." Genes 4, no. 2: 264-274.