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Comparison of the Fecal Microbiota in Feral and Domestic Goats
Microbial Ecology Laboratory, JGD 224, Department of Biology, University of Puerto Rico, Río Piedras Campus, Ave Ponce de León, San Juan, PR 00931, USA
Department of Ecology, Earth Sciences Division, Lawrence Berkley National Laboratory, 1 Cyclotron Rd., MS 90-1116, Berkley, CA 94720, USA
Current address: DOE-Joint Genome Institute, 2800 Mitchell Dr., Walnut Creek, CA 94598, USA.
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 2 November 2011; in revised form: 22 November 2011 / Accepted: 28 November 2011 / Published: 21 December 2011
Abstract: Animals have co-evolved with mutualistic microbial communities, known as the microbiota, which are essential for organ development and function. We hypothesize that modern animal husbandry practices exert an impact on the intestinal microbiota. In this study, we compared the structure of the fecal microbiota between feral and domestic goats using the G2 PhyloChip and assessed the presence of five tetracycline resistance genes [tet(M), tet(S), tet(O), tet(Q) and tet(W)] by PCR. Feces were collected from 10 goats: 5 domestic from a farm in the main island of Puerto Rico and 5 feral from the remote dry island of Mona. There were 42 bacterial phyla from 153 families detected in the goats’ feces. A total of 84 PhyloChip-OTUs were different in the fecal microbiota of feral and domestic goat. Both feral and domestic goats carried antibiotic resistance genes tet(O) and tet(W), but domestic goats additionally carried tet(Q). Diet, host genetics and antibiotic exposure are likely determinant factors in shaping the intestinal microbiota and may explain the differences observed between feral and domestic goats fecal microbiota.
Keywords: feral; domestic; microbiome; antibiotic; resistance
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De Jesús-Laboy, K.M.; Godoy-Vitorino, F.; Piceno, Y.M.; Tom, L.M.; Pantoja-Feliciano, I.G.; Rivera-Rivera, M.J.; Andersen, G.L.; Domínguez-Bello, M.G. Comparison of the Fecal Microbiota in Feral and Domestic Goats. Genes 2012, 3, 1-18.
De Jesús-Laboy KM, Godoy-Vitorino F, Piceno YM, Tom LM, Pantoja-Feliciano IG, Rivera-Rivera MJ, Andersen GL, Domínguez-Bello MG. Comparison of the Fecal Microbiota in Feral and Domestic Goats. Genes. 2012; 3(1):1-18.
De Jesús-Laboy, Kassandra M.; Godoy-Vitorino, Filipa; Piceno, Yvette M.; Tom, Lauren M.; Pantoja-Feliciano, Ida G.; Rivera-Rivera, Michelle J.; Andersen, Gary L.; Domínguez-Bello, María G. 2012. "Comparison of the Fecal Microbiota in Feral and Domestic Goats." Genes 3, no. 1: 1-18.