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Genes 2011, 2(4), 788-803; doi:10.3390/genes2040788

Identification of Genes Involved in the Glycosylation of Modified Viosamine of Flagellins in Pseudomonas syringae by Mass Spectrometry

1
National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
2
The Graduate School of Natural Science and Technology, Okayama University, Tsushima-naka 1-1-1, Okayama 700-8530, Japan
*
Author to whom correspondence should be addressed.
Received: 2 September 2011 / Revised: 20 October 2011 / Accepted: 21 October 2011 / Published: 28 October 2011
(This article belongs to the Special Issue Genes and Genomes of Plant Pathogenic Bacteria)
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Abstract

Previously we revealed that flagellin proteins in Pseudomonas syringae pv. tabaci 6605 (Pta 6605) were glycosylated with a trisaccharide, modified viosamine (mVio)-rhamnose-rhamnose and that glycosylation was required for virulence. We further identified some glycosylation-related genes, including vioA, vioB, vioT, fgt1, and fgt2. In this study, we newly identified vioR and vioM in a so-called viosamine island as biosynthetic genes for glycosylation of mVio in Pta 6605 by the mass spectrometry (MS) of flagellin glycan in the respective mutants. Furthermore, characterization of the mVio-related genes and MS analyses of flagellin glycans in other pathovars of P. syringae revealed that mVio-related genes were essential for mVio biosynthesis in flagellin glycans, and that P. syringae pv. syringae B728a, which does not possess a viosamine island, has a different structure of glycan in its flagellin protein.
Keywords: flagellin; glycosylation; mass spectrometry; viosamine island flagellin; glycosylation; mass spectrometry; viosamine island
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MDPI and ACS Style

Yamamoto, M.; Ohnishi-Kameyama, M.; Nguyen, C.L.; Taguchi, F.; Chiku, K.; Ishii, T.; Ono, H.; Yoshida, M.; Ichinose, Y. Identification of Genes Involved in the Glycosylation of Modified Viosamine of Flagellins in Pseudomonas syringae by Mass Spectrometry. Genes 2011, 2, 788-803.

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