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Complete Genome Sequence of the Soybean Symbiont Bradyrhizobium japonicum Strain USDA6T
Faculty of Engineering, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603-8555, Japan
Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-Ku, Kyoto 603-8555, Japan
Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba 292-0818, Japan
Graduate School of Life Sciences, Tohoku University, Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan
* Author to whom correspondence should be addressed.
Received: 7 September 2011; in revised form: 11 October 2011 / Accepted: 12 October 2011 / Published: 28 October 2011
Abstract: The complete nucleotide sequence of the genome of the soybean symbiont Bradyrhizobium japonicum strain USDA6T was determined. The genome of USDA6T is a single circular chromosome of 9,207,384 bp. The genome size is similar to that of the genome of another soybean symbiont, B. japonicum USDA110 (9,105,828 bp). Comparison of the whole-genome sequences of USDA6T and USDA110 showed colinearity of major regions in the two genomes, although a large inversion exists between them. A significantly high level of sequence conservation was detected in three regions on each genome. The gene constitution and nucleotide sequence features in these three regions indicate that they may have been derived from a symbiosis island. An ancestral, large symbiosis island, approximately 860 kb in total size, appears to have been split into these three regions by unknown large-scale genome rearrangements. The two integration events responsible for this appear to have taken place independently, but through comparable mechanisms, in both genomes.
Keywords: genome sequence; symbiosis island; soybean; genomic island; insertion sequence; nodulation; Bradyrhizobium; microsymbiont
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Kaneko, T.; Maita, H.; Hirakawa, H.; Uchiike, N.; Minamisawa, K.; Watanabe, A.; Sato, S. Complete Genome Sequence of the Soybean Symbiont Bradyrhizobium japonicum Strain USDA6T. Genes 2011, 2, 763-787.
Kaneko T, Maita H, Hirakawa H, Uchiike N, Minamisawa K, Watanabe A, Sato S. Complete Genome Sequence of the Soybean Symbiont Bradyrhizobium japonicum Strain USDA6T. Genes. 2011; 2(4):763-787.
Kaneko, Takakazu; Maita, Hiroko; Hirakawa, Hideki; Uchiike, Nobukazu; Minamisawa, Kiwamu; Watanabe, Akiko; Sato, Shusei. 2011. "Complete Genome Sequence of the Soybean Symbiont Bradyrhizobium japonicum Strain USDA6T." Genes 2, no. 4: 763-787.