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Cells 2017, 6(3), 18; doi:10.3390/cells6030018

Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum

1
Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain
2
Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico
3
Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland
4
Department of Biomedical Sciences and Bateson Centre, University of Sheffield, Sheffield S10 2TN, UK
*
Author to whom correspondence should be addressed.
Received: 22 May 2017 / Revised: 27 June 2017 / Accepted: 28 June 2017 / Published: 3 July 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
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Abstract

Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism. View Full-Text
Keywords: autophagy; Dictyostelium; flux assays; autophagic markers; cleavage assays autophagy; Dictyostelium; flux assays; autophagic markers; cleavage assays
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Domínguez-Martín, E.; Cardenal-Muñoz, E.; King, J.S.; Soldati, T.; Coria, R.; Escalante, R. Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum. Cells 2017, 6, 18.

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