Next Article in Journal / Special Issue
Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum
Previous Article in Journal / Special Issue
Assessing Autophagy in Mouse Models and Patients with Systemic Autoimmune Diseases
Article Menu

Export Article

Open AccessFeature PaperReview
Cells 2017, 6(3), 17; doi:10.3390/cells6030017

Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue

Laboratory of Physiopharmacology, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium
*
Author to whom correspondence should be addressed.
Received: 24 May 2017 / Revised: 20 June 2017 / Accepted: 26 June 2017 / Published: 30 June 2017
(This article belongs to the Special Issue Assays to Monitor Autophagy in Model Systems)
View Full-Text   |   Download PDF [4852 KB, uploaded 30 June 2017]   |  

Abstract

Autophagy is a highly conserved lysosomal degradation pathway with major impact on diverse human pathologies. Despite the development of different methodologies to detect autophagy both in vitro and in vivo, monitoring autophagy in tissue via immunohistochemical techniques is hampered due to the lack of biomarkers. Immunohistochemical detection of a punctate pattern of ATG8/MAP1LC3 proteins is currently the most frequently used approach to detect autophagy in situ, but it depends on a highly sensitive detection method and is prone to misinterpretation. Moreover, reliable MAP1LC3 immunohistochemical staining requires correct tissue processing and high-quality, isoform-specific antibodies. Immunohistochemical analysis of other autophagy-related protein targets such as SQSTM1, ubiquitin, ATG5 or lysosomal proteins is not recommended as marker for autophagic activity in tissue for multiple reasons including aspecific labeling of cellular structures and a lack of differential protein expression during autophagy initiation. To better understand the role of autophagy in human disease, novel biomarkers for visualization of the autophagic process with standard histology techniques are urgently needed. View Full-Text
Keywords: autophagy; immunohistochemistry; MAP1LC3; SQSTM1; autophagosome autophagy; immunohistochemistry; MAP1LC3; SQSTM1; autophagosome
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Martinet, W.; Roth, L.; De Meyer, G.R.Y. Standard Immunohistochemical Assays to Assess Autophagy in Mammalian Tissue. Cells 2017, 6, 17.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top