Cells 2014, 3(2), 500-516; doi:10.3390/cells3020500
Article

Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

1,* email, 1email, 1email, 2email, 3email and 4email
Received: 10 March 2014; in revised form: 7 May 2014 / Accepted: 14 May 2014 / Published: 23 May 2014
(This article belongs to the Special Issue Transient Receptor Potential (TRP) Channels)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The transient receptor potential melastatin-subfamily member 8 (TRPM8) channels control Ca2+ homeostasis. Recent studies indicate that TRPM8 channels are aberrantly expressed and required for cellular proliferation in pancreatic adenocarcinoma. However, the functional significance of TRPM8 in pancreatic tissues is mostly unknown. The objectives of this study are to examine the expression of TRPM8 in various histopathological types of pancreatic tissues, determine its clinical significance in pancreatic adenocarcinoma, and investigate its functional role in cancer cells invasion. We present evidence that, in normal pancreatic tissues, anti-TRPM8 immunoreactivity is detected in the centroacinar cells and the islet endocrine cells. In pre-malignant pancreatic tissues and malignant neoplasms, TRPM8 is aberrantly expressed to variable extents. In the majority of pancreatic adenocarcinoma, TRPM8 is expressed at moderate or high levels, and anti-TRPM8 immunoreactivity positively correlates with the primary tumor size and stage. In the pancreatic adenocarcinoma cell lines that express relatively high levels of TRPM8, short hairpin RNA-mediated interference of TRPM8 expression impaired their ability of invasion. These data suggest that aberrantly expressed TRPM8 channels play contributory roles in pancreatic tumor growth and metastasis, and support exploration of TRPM8 as a biomarker and target of pancreatic adenocarcinoma.
Keywords: biomarker; cell invasion; cell migration; ion channel; molecular target; pancreatic cancer; TRPM8; transient receptor potential
PDF Full-text Download PDF Full-Text [1463 KB, uploaded 23 May 2014 13:38 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Yee, N.S.; Li, Q.; Kazi, A.A.; Yang, Z.; Berg, A.; Yee, R.K. Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion. Cells 2014, 3, 500-516.

AMA Style

Yee NS, Li Q, Kazi AA, Yang Z, Berg A, Yee RK. Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion. Cells. 2014; 3(2):500-516.

Chicago/Turabian Style

Yee, Nelson S.; Li, Qin; Kazi, Abid A.; Yang, Zhaohai; Berg, Arthur; Yee, Rosemary K. 2014. "Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion." Cells 3, no. 2: 500-516.

Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert