Next Article in Journal
Functional Assessment of Pharmacological Telomerase Activators in Human T Cells
Next Article in Special Issue
Cardiomyocyte Regeneration
Previous Article in Journal
CD8+ T Lymphocyte Epitopes From The Herpes Simplex Virus Type 2 ICP27, VP22 and VP13/14 Proteins To Facilitate Vaccine Design And Characterization
Previous Article in Special Issue
The “Stars and Stripes” Metaphor for Animal Regeneration-Elucidating Two Fundamental Strategies along a Continuum
Cells 2013, 2(1), 43-56; doi:10.3390/cells2010043
Review

Redirection of Human Cancer Cells upon the Interaction with the Regenerating Mouse Mammary Gland Microenvironment

 and *
Received: 4 December 2012; in revised form: 24 December 2012 / Accepted: 8 January 2013 / Published: 10 January 2013
(This article belongs to the Special Issue Tissue and Organ Regeneration)
View Full-Text   |   Download PDF [828 KB, uploaded 10 January 2013]   |   Browse Figures
Abstract: Tumorigenesis is often described as a result of accumulated mutations that lead to growth advantage and clonal expansion of mutated cells. There is evidence in the literature that cancer cells are influenced by the microenvironment. Our previous studies demonstrated that the mouse mammary gland is capable of redirecting mouse cells of non-mammary origins as well as Mouse Mammary Tumor Virus (MMTV)-neu transformed cells toward normal mammary epithelial cell fate during gland regeneration. Interestingly, the malignant phenotype of MMTV-neu transformed cells was suppressed during serial transplantation experiments. Here, we discuss our studies that demonstrated the potential of the regenerating mouse mammary gland to redirect cancer cells of different species into a functional tumor-free mammary epithelial cell progeny. Immunochemistry for human specific CD133, mitochondria, cytokeratins as well as milk proteins and FISH for human specific probe identified human epithelial cell progeny in ducts, lobules, and secretory acini. Fluorescent In Situ Hybridization (FISH) for human centromeric DNA and FACS analysis of propidium iodine staining excluded the possibility of mouse-human cell fusion. To our knowledge this is the first evidence that human cancer cells of embryonic or somatic origins respond to developmental signals generated by the mouse mammary gland microenvironment during gland regeneration in vivo.
Keywords: human cancer cells; redirecting cell fate; progenitor cell; mammary gland; microenvironment human cancer cells; redirecting cell fate; progenitor cell; mammary gland; microenvironment
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Rosenfield, S.M.; Smith, G.H. Redirection of Human Cancer Cells upon the Interaction with the Regenerating Mouse Mammary Gland Microenvironment. Cells 2013, 2, 43-56.

AMA Style

Rosenfield SM, Smith GH. Redirection of Human Cancer Cells upon the Interaction with the Regenerating Mouse Mammary Gland Microenvironment. Cells. 2013; 2(1):43-56.

Chicago/Turabian Style

Rosenfield, Sonia M.; Smith, Gilbert H. 2013. "Redirection of Human Cancer Cells upon the Interaction with the Regenerating Mouse Mammary Gland Microenvironment." Cells 2, no. 1: 43-56.


Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert