Next Article in Journal
Previous Article in Journal
Cells 2013, 2(1), 57-66; doi:10.3390/cells2010057
Article

Functional Assessment of Pharmacological Telomerase Activators in Human T Cells

1
, 1
, 1
, 1
, 1
, 2
 and 1,*
Received: 27 August 2012; in revised form: 7 January 2013 / Accepted: 8 January 2013 / Published: 14 January 2013
View Full-Text   |   Download PDF [658 KB, uploaded 14 January 2013]
Abstract: Telomeres are structures at the ends of chromosomes that shorten during cell division and eventually signal an irreversible state of growth arrest known as cellular senescence. To delay this cellular aging, human T cells, which are critical in the immune control over infections and cancer, activate the enzyme telomerase, which binds and extends the telomeres. Several different extracts from the Astragalus membranaceus root have been documented to activate telomerase activity in human T cells. The objective of this research was to compare two extracts from Astragalus membranaceus, TA-65 and HTA, for their effects on both telomerase and proliferative activity of human CD4 and CD8 T cells. Our results demonstrate that, TA-65 increased telomerase activity significantly (1.3 to 3.3-fold relative to controls) in T cell cultures from six donors tested, whereas HTA only increased telomerase levels in two out of six donors. We also demonstrate that TA-65 activates telomerase by a MAPK- specific pathway. Finally, we determine that during a three-day culture period, only the T cells treated with the TA-65 extract showed a statistically significant increase in proliferative activity. Our results underscore the importance of comparing multiple telomerase activators within the same experiment, and of including functional assays in addition to measuring telomerase activity.
Keywords: TA-65; telomerase activators; cellular senescence TA-65; telomerase activators; cellular senescence
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Molgora, B.; Bateman, R.; Sweeney, G.; Finger, D.; Dimler, T.; Effros, R.B.; Valenzuela, H.F. Functional Assessment of Pharmacological Telomerase Activators in Human T Cells. Cells 2013, 2, 57-66.

AMA Style

Molgora B, Bateman R, Sweeney G, Finger D, Dimler T, Effros RB, Valenzuela HF. Functional Assessment of Pharmacological Telomerase Activators in Human T Cells. Cells. 2013; 2(1):57-66.

Chicago/Turabian Style

Molgora, Brenda; Bateman, Riley; Sweeney, Greg; Finger, Danielle; Dimler, Taylor; Effros, Rita B.; Valenzuela, Hector F. 2013. "Functional Assessment of Pharmacological Telomerase Activators in Human T Cells." Cells 2, no. 1: 57-66.


Cells EISSN 2073-4409 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert