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Cells 2012, 1(4), 1121-1132; doi:10.3390/cells1041121
Article
Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals
Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Dr. 303A, Bethesda 20892-4340, MD, USA
* Author to whom correspondence should be addressed.
Received: 16 October 2012; in revised form: 6 November 2012 / Accepted: 7 November 2012 / Published: 16 November 2012
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
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Abstract: Intravital microscopy has enabled imaging of the dynamics of subcellular structures in live animals, thus opening the door to investigating membrane trafficking under physiological conditions. Here, we sought to determine whether the architecture and the environment of a fully developed tissue influences the dynamics of endocytic processes. To this aim, we imaged endocytosis in the stromal cells of rat salivary glands both in situ and after they were isolated and cultured on a solid surface. We found that the internalization of transferrin and dextran, two molecules that traffic via distinct mechanisms, is substantially altered in cultured cells, supporting the idea that the three dimensional organization of the tissue and the cues generated by the surrounding environment strongly affect membrane trafficking events.
Keywords: intravital microscopy; in vivo imaging; endocytosis; cytoskeleton; salivary glands
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MDPI and ACS Style
Masedunskas, A.; Porat-Shliom, N.; Rechache, K.; Aye, M.-P.; Weigert, R. Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals. Cells 2012, 1, 1121-1132.
AMA StyleMasedunskas A, Porat-Shliom N, Rechache K, Aye M-P, Weigert R. Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals. Cells. 2012; 1(4):1121-1132.
Chicago/Turabian StyleMasedunskas, Andrius; Porat-Shliom, Natalie; Rechache, Kamil; Aye, Myo-Pale'; Weigert, Roberto. 2012. "Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals." Cells 1, no. 4: 1121-1132.