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Cells 2012, 1(4), 1121-1132; doi:10.3390/cells1041121
Article

Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals

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Received: 16 October 2012; in revised form: 6 November 2012 / Accepted: 7 November 2012 / Published: 16 November 2012
(This article belongs to the Special Issue Imaging in Cell Biology and Development)
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Abstract: Intravital microscopy has enabled imaging of the dynamics of subcellular structures in live animals, thus opening the door to investigating membrane trafficking under physiological conditions. Here, we sought to determine whether the architecture and the environment of a fully developed tissue influences the dynamics of endocytic processes. To this aim, we imaged endocytosis in the stromal cells of rat salivary glands both in situ and after they were isolated and cultured on a solid surface. We found that the internalization of transferrin and dextran, two molecules that traffic via distinct mechanisms, is substantially altered in cultured cells, supporting the idea that the three dimensional organization of the tissue and the cues generated by the surrounding environment strongly affect membrane trafficking events.
Keywords: intravital microscopy; in vivo imaging; endocytosis; cytoskeleton; salivary glands intravital microscopy; in vivo imaging; endocytosis; cytoskeleton; salivary glands
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Masedunskas, A.; Porat-Shliom, N.; Rechache, K.; Aye, M.-P.; Weigert, R. Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals. Cells 2012, 1, 1121-1132.

AMA Style

Masedunskas A, Porat-Shliom N, Rechache K, Aye M-P, Weigert R. Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals. Cells. 2012; 1(4):1121-1132.

Chicago/Turabian Style

Masedunskas, Andrius; Porat-Shliom, Natalie; Rechache, Kamil; Aye, Myo-Pale'; Weigert, Roberto. 2012. "Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals." Cells 1, no. 4: 1121-1132.



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