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Agronomy 2016, 6(1), 12; doi:10.3390/agronomy6010012

Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development

1
Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay, RD10, 78026 Versailles Cedex, France
2
The University of Tokyo, Department of Applied Biological Chemistry, Yayoi l-1-1, Bunkyo-ku, 113-8657 Tokyo, Japan
3
Osaka University, Institute for Protein Research, Suita, Osaka 565-0871, Japan
4
INRA, IJPB, UMR1318, ERL CNRS 3559, Saclay Plant Sciences, RD10, F-78026 Versailles, France
*
Author to whom correspondence should be addressed.
Academic Editors: Anne Krapp and Bertrand Hirel
Received: 22 October 2015 / Revised: 28 January 2016 / Accepted: 4 February 2016 / Published: 14 February 2016
(This article belongs to the Special Issue Nitrogen Transport and Assimilation in Plants)
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Abstract

The aim of this study was to investigate the role of ASN3-encoded asparagine synthetase (AS, EC 6.3.5.4) during vegetative growth, seed development and germination of Arabidopsis thaliana. Phenotypic analysis of knockout (asn3-1) and knockdown (asn3-2) T-DNA insertion mutants for the ASN3 gene (At5g10240) demonstrated wild-type contents of asparagine synthetase protein, chlorophyll and ammonium in green leaves at 35 days after sowing. In situ hybridization localized ASN3 mRNA to phloem companion cells of vasculature. Young siliques of the asn3-1 knockout line showed a decrease in asparagine but an increase in glutamate. The seeds of asn3-1 and asn3-2 displayed a wild-type nitrogen status expressed as total nitrogen content, indicating that the repression of ASN3 expression had only a limited effect on mature seeds. An analysis of amino acid labeling of seeds imbibed with (15N) ammonium for 24 h revealed that asn3-1 seeds contained 20% less total asparagine while 15N-labeled asparagine ((2-15N)asparagine, (4-15N)asparagine and (2,4-15N)asparagine) increased by 12% compared to wild-type seeds. The data indicate a fine regulation of asparagine synthesis and hydrolysis in Arabidopsis seeds. View Full-Text
Keywords: amino acids; Arabidopsis thaliana; asparagine synthetase; nitrogen metabolism; seed nitrogen amino acids; Arabidopsis thaliana; asparagine synthetase; nitrogen metabolism; seed nitrogen
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gaufichon, L.; Marmagne, A.; Yoneyama, T.; Hase, T.; Clément, G.; Trassaert, M.; Xu, X.; Shakibaei, M.; Najihi, A.; Suzuki, A. Impact of the Disruption of ASN3-Encoding Asparagine Synthetase on Arabidopsis Development. Agronomy 2016, 6, 12.

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