Agronomy 2013, 3(4), 794-815; doi:10.3390/agronomy3040794
Article

Catch the Best: Novel Screening Strategy to Select Stress Protecting Agents for Crop Plants

Received: 23 August 2013; in revised form: 13 November 2013 / Accepted: 19 November 2013 / Published: 26 November 2013
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Climate change increases stress levels for crops and affects the economic and environmental aspects of agricultural management systems. The application of stress tolerance-mediating microorganisms is an auspicious strategy for improving crop protection, and as such, we developed a direct selection strategy to obtain cultivable microorganisms from promising bioresources using the bait plants, maize, oilseed rape, sorghum and sugar beet. Alpine mosses, lichens and primrose were selected as bioresources, as each is adapted to adverse environmental conditions. A 10% crop-specific selection was found for bait plant rhizosphere communities using cultivation-independent fingerprints, and their potential role as stress protecting agents (SPA) was evaluated following the cultivation of captured bacteria. In addition to assays identifying phytopathogen antagonism and plant growth promotion capacities, our evaluation included those that test the ability to allocate nutrients. Moreover, we developed new assays to measure tolerance in diverse stress conditions. A score scheme was applied to select SPAs with desired properties, and three Pseudomonas species with pronounced antagonistic activity that showed elevated tolerance to desiccation and an improved seed germination rate were subsequently chosen. Screening for environmentally-conditioned and host-adapted microorganisms provides a novel tool for target-oriented exploitation of microbial bioresources for the management of ecofriendly crops facing biotic and abiotic stresses.
Keywords: bait plants; biological control agents (BCAs); stress protecting agents (SPAs); screening; abiotic stress
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MDPI and ACS Style

Zachow, C.; Müller, H.; Tilcher, R.; Donat, C.; Berg, G. Catch the Best: Novel Screening Strategy to Select Stress Protecting Agents for Crop Plants. Agronomy 2013, 3, 794-815.

AMA Style

Zachow C, Müller H, Tilcher R, Donat C, Berg G. Catch the Best: Novel Screening Strategy to Select Stress Protecting Agents for Crop Plants. Agronomy. 2013; 3(4):794-815.

Chicago/Turabian Style

Zachow, Christin; Müller, Henry; Tilcher, Ralf; Donat, Christina; Berg, Gabriele. 2013. "Catch the Best: Novel Screening Strategy to Select Stress Protecting Agents for Crop Plants." Agronomy 3, no. 4: 794-815.

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