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Polymers 2016, 8(5), 175; doi:10.3390/polym8050175

Polyurethane/Polylactide-Blend Films Doped with Zinc Ions for the Growth and Expansion of Human Olfactory Ensheathing Cells (OECs) and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) for Regenerative Medicine Applications

1
Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, Wroclaw 51-631, Poland
2
Department of Animal Physiology and Biostructure, Wroclaw University of Environmental and Life Sciences, Wroclaw 50-375, Poland
3
Electron Microscopy Laboratory, Wroclaw Research Centre EIT+, Wroclaw 54-066, Poland
4
Department of Neurosurgery, Lower Silesia Specialist Hospital of T. Marciniak, Emergency Medicine Centre, Wroclaw 54-049, Poland
5
Faculty of Materials Science and Ceramics, AGH University of Science and Technology, Krakow 30-059, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Jianxun Ding
Received: 29 February 2016 / Revised: 6 April 2016 / Accepted: 8 April 2016 / Published: 30 April 2016
(This article belongs to the Special Issue Functional Polymers for Medical Applications)
View Full-Text   |   Download PDF [10895 KB, uploaded 30 April 2016]   |  

Abstract

Polymeric biomaterials based on polyurethane and polylactide blends are promising candidates for regenerative medicine applications as biocompatible, bioresorbable carriers. In current research we showed that 80/20 polyurethane/polylactide blends (PU/PLDL) with confirmed biological properties in vitro may be further improved by the addition of ZnO nanoparticles for the delivery of bioactive zinc oxide for cells. The PU/PLDL blends were doped with different concentrations of ZnO (0.001%, 0.01%, 0.05%) and undertaken for in vitro biological evaluation using human adipose stromal stem cells (ASCs) and olfactory ensheathing cells (OECs). The addition of 0.001% of ZnO to the biomaterials positively influenced the morphology, proliferation, and phenotype of cells cultured on the scaffolds. Moreover, the analysis of oxidative stress markers revealed that 0.001% of ZnO added to the material decreased the stress level in both cell lines. In addition, the levels of neural-specific genes were upregulated in OECs when cultured on sample 0.001 ZnO, while the apoptosis-related genes were downregulated in OECs and ASCs in the same group. Therefore, we showed that PU/PLDL blends doped with 0.001% of ZnO exert beneficial influence on ASCs and OECs in vitro and they may be considered for future applications in the field of regenerative medicine. View Full-Text
Keywords: polyurethane; polylactide; zinc oxide; adipose stromal stem cells; olfactory ensheathing cells polyurethane; polylactide; zinc oxide; adipose stromal stem cells; olfactory ensheathing cells
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Marycz, K.; Marędziak, M.; Grzesiak, J.; Szarek, D.; Lis, A.; Laska, J. Polyurethane/Polylactide-Blend Films Doped with Zinc Ions for the Growth and Expansion of Human Olfactory Ensheathing Cells (OECs) and Adipose-Derived Mesenchymal Stromal Stem Cells (ASCs) for Regenerative Medicine Applications. Polymers 2016, 8, 175.

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