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Polymers 2016, 8(5), 178; doi:10.3390/polym8050178

Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers

1
Faculty of Natural Science II, Martin-Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, D-06120 Halle (Saale), Germany
2
Institute for Medical Physics and Biophysics, Leipzig University, Härtelstraße 16-18, D-04107 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: André Laschewsky
Received: 31 March 2016 / Revised: 25 April 2016 / Accepted: 26 April 2016 / Published: 2 May 2016
(This article belongs to the Special Issue Polymers for Aqueous Media)
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Abstract

The formation of amyloid fibrils is considered to be one of the main causes for many neurodegenerative diseases, such as Alzheimer’s, Parkinson’s or Huntington’s disease. Current knowledge suggests that amyloid-aggregation represents a nucleation-dependent aggregation process in vitro, where a sigmoidal growth phase follows an induction period. Here, we studied the fibrillation of amyloid β 1-40 (Aβ40) in the presence of thermoresponsive polymers, expected to alter the Aβ40 fibrillation kinetics due to their lower critical solution behavior. To probe the influence of molecular weight and the end groups of the polymer on its lower critical solution temperature (LCST), also considering its concentration dependence in the presence of buffer-salts needed for the aggregation studies of the amyloids, poly(oxazolines) (POx) with LCSTs ranging from 14.2–49.8 °C and poly(methoxy di(ethylene glycol)acrylates) with LCSTs ranging from 34.4–52.7 °C were synthesized. The two different polymers allowed the comparison of the influence of different molecular structures onto the fibrillation process. Mixtures of Aβ40 with these polymers in varying concentrations were studied via time-dependent measurements of the thioflavin T (ThT) fluorescence. The studies revealed that amyloid fibrillation was accelerated in, accompanied by an extension of the lag phase of Aβ40 fibrillation from 18.3 h in the absence to 19.3 h in the presence of the poly(methoxy di(ethylene glycol)acrylate) (3600 g/mol). View Full-Text
Keywords: amyloid β; fibrillation; lower critical solution temperature; thermoresponsive polymer amyloid β; fibrillation; lower critical solution temperature; thermoresponsive polymer
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Funtan, S.; Evgrafova, Z.; Adler, J.; Huster, D.; Binder, W.H. Amyloid Beta Aggregation in the Presence of Temperature-Sensitive Polymers. Polymers 2016, 8, 178.

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