Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability
Abstract
:Abbreviations
CKC | Canine Kidney Cells |
CRBC | Chicken Red Blood Cells |
CV | Coefficient of Variation |
DI | DNA Index |
FCM | Flow Cytometry |
HS | Histiocytic Sarcoma |
MTB | Malignant Tumor of Bone |
OS | Osteosarcoma |
PD | Peridiploid |
STS | Soft Tissue Sarcoma |
1. Introduction
2. Results
2.1. DNA-Ploidy Status in Primary Lesions
Subtype | Diploid (n) | Aneuploid (n) | Peridiploid (n) | Total (n) |
---|---|---|---|---|
Malignant tumors of bone | ||||
Osteosarcoma | 9 | 14 | 3 | 26 |
Chondrosarcoma | 1 | 2 | - | 3 |
Multilobular tumor of bone | 0 | 1 | - | 1 |
Total | 10 | 17 | 3 | 30 |
Soft tissue sarcomas | ||||
Fibrosarcoma | 2 | 1 | - | 3 |
Sarcoma-not otherwise specified | 3 | 1 | - | 4 |
Rhabdomyosarcoma | 2 | 3 | - | 5 |
Malignant peripheral nerve sheath tumors | 7 | 1 | - | 8 |
Synovial sarcoma | 0 | 5 | - | 5 |
Liposarcoma | 0 | 3 | - | 3 |
Leiomyosarcoma | 6 | 2 | - | 8 |
Hemangiosarcoma | 1 | 3 | - | 4 |
Histiocytic sarcoma | 0 | 6 | 1 | 7 |
Total | 21 | 25 | 1 | 47 |
Tumor type and grade | Diploid (n) | Peridiploid (n) | Aneuploid (n) |
---|---|---|---|
Malignant tumor of bone | |||
-grade I | 0 | 0 | 0 |
-grade II | 1 | 1 | 2 |
-grade III | 8 | 2 | 12 |
Soft tissue sarcoma | |||
-grade I | 5 | 0 | 2 |
-grade II | 5 | 0 | 2 |
-grade III | 8 | 0 | 11 |
Diploid | Aneuploid | Peridiploid | |
---|---|---|---|
P53-wt | 12 | 14 | 2 |
P53- alteration | 7 | 9 | 1 |
Tumor group | Ploidy status | |
---|---|---|
Diploid | Aneuploid | |
Osteosarcomas | ||
-Metastases | 8 | 12 |
-No metastases | 0 | 0 |
Soft tissue sarcomas | ||
-Metastases | 5 | 17 |
-No metastases | 8 | 3 |
2.2. Heterogeneity of Ploidy Status or DI
Case | Primary | Met. 1 | Met. 2 | Met. 3 | Met. 4 |
---|---|---|---|---|---|
Osteosarcoma 1 * | 2.10 | 1.10/2.14 | 1.12 | ||
Osteosarcoma 2 * | 0.76 | 0.77/1.56 | 0.79/1.43/1.59 | 0.79/1.55 | 0.78/1.55 |
Osteosarcoma 3 * | 1.0 | 1.88 | |||
Osteosarcoma 4 | 1.0 | 1.0 | 1.0 | ||
Synovial cell sarcoma * | 0.89/1.80 | 1.77 | |||
Hemangiosarcoma * | 1.89 | 1.0 | 1.0 | ||
Sarcoma-NOS * | 1.0 | 1.77 | |||
Fibrosarcoma | 1.0 | 1.0 | 1.0 | ||
Malignant Peripheral Nerve Sheet Tumor | 1.0 | 1.0 | |||
Synovial Cell Sarcoma | 0.72 | 0.72 | |||
Histiocytic Sarcoma | PD | 0.93 | |||
Liposarcoma | 2.03 | 1.97 |
3. Discussion
4. Experimental Section
4.1. Animals
4.2. Preparation of Samples
4.3. Histological Examination
4.4. Flow Cytometry Analysis of Nuclear DNA Content
4.5. DNA-Ploidy Assessment
4.6. Mutational Analysis of the p53 Gene
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Boerkamp, K.M.; Rutteman, G.R.; Kik, M.J.L.; Kirpensteijn, J.; Schulze, C.; Grinwis, G.C.M. Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability. Cancers 2012, 4, 1300-1317. https://doi.org/10.3390/cancers4041300
Boerkamp KM, Rutteman GR, Kik MJL, Kirpensteijn J, Schulze C, Grinwis GCM. Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability. Cancers. 2012; 4(4):1300-1317. https://doi.org/10.3390/cancers4041300
Chicago/Turabian StyleBoerkamp, Kim M., Gerard R. Rutteman, Marja J. L. Kik, Jolle Kirpensteijn, Christoph Schulze, and Guy C. M. Grinwis. 2012. "Nuclear DNA-Content in Mesenchymal Lesions in Dogs: Its Value as Marker of Malignancy and Extent of Genomic Instability" Cancers 4, no. 4: 1300-1317. https://doi.org/10.3390/cancers4041300