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Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
Laboratory for Translational Brain Tumor and Stem Cell Research, Department of Neurological Surgery, Weill Cornell Brain Tumor Center, Weill Cornell Medical College, New York, NY 10021, USA
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Received: 4 March 2011; in revised form: 4 May 2011 / Accepted: 31 May 2011 / Published: 10 June 2011
Abstract: The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for optimizing the derivation and culturing of stem-like cells also known as tumor stem cells (TSCs) from patient-derived GBM tissue samples. The hallmarks of TSCs are that they must be able to self-renew and retain tumorigenicity. The isolation, optimization and derivation of TSCs as outlined in this review, will be important in understanding biology and therapeutic applications related to these cells.
Keywords: glioblastoma multiforme; stem-like cells; brain tumor stem cells; differentiation; culturing; stem-cell hypothesis
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Gürsel, D.B.; Shin, B.J.; Burkhardt, J.-K.; Kesavabhotla, K.; Schlaff, C.D.; Boockvar, J.A. Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications. Cancers 2011, 3, 2655-2666.
Gürsel DB, Shin BJ, Burkhardt J-K, Kesavabhotla K, Schlaff CD, Boockvar JA. Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications. Cancers. 2011; 3(2):2655-2666.
Gürsel, Demirkan B.; Shin, Benjamin J.; Burkhardt, Jan-Karl; Kesavabhotla, Kartik; Schlaff, Cody D.; Boockvar, John A. 2011. "Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications." Cancers 3, no. 2: 2655-2666.