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Cancers 2010, 2(1), 133-142; doi:10.3390/cancers2010133
Article

Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma

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Received: 27 January 2010; in revised form: 8 February 2010 / Accepted: 20 February 2010 / Published: 1 March 2010
(This article belongs to the Special Issue Biomarkers: Oncology Studies)
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Abstract: To identify biomarkers for early detection for esophageal squamous cell carcinoma (ESCC), we previously carried out a genome-wide gene expression profiling study using an oligonucleotide microarray platform. This analysis led to identification of several transcripts that were significantly upregulated in ESCC compared to the adjacent normal epithelium. In the current study, we performed immunohistochemical analyses of protein products for two candidates genes identified from the DNA microarray analysis, periostin (POSTN) and lumican (LUM), using tissue microarrays. Increased expression of both periostin and lumican was observed in 100% of 137 different ESCC samples arrayed on tissue microarrays. Increased expression of periostin and lumican was observed in carcinoma as well as in stromal cell in the large majority of cases. These findings suggest that these candidates can be investigated in the sera of ESCC patients using ELISA or multiple reaction monitoring (MRM) type assays to further explore their utility as biomarkers.
Keywords: stroma; extracellular matrix; DNA microarrays; epithelial-mesenchymal transition stroma; extracellular matrix; DNA microarrays; epithelial-mesenchymal transition
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kashyap, M.K.; Marimuthu, A.; Peri, S.; Kumar, G.S.S.; Jacob, H.K.; Prasad, T.S.K.; Mahmood, R.; Kumar, K.V.V.; Kumar, M.V.; Meltzer, S.J.; Montgomery, E.A.; Kumar, R.V.; Pandey, A. Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma. Cancers 2010, 2, 133-142.

AMA Style

Kashyap MK, Marimuthu A, Peri S, Kumar GSS, Jacob HK, Prasad TSK, Mahmood R, Kumar KVV, Kumar MV, Meltzer SJ, Montgomery EA, Kumar RV, Pandey A. Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma. Cancers. 2010; 2(1):133-142.

Chicago/Turabian Style

Kashyap, Manoj Kumar; Marimuthu, Arivusudar; Peri, Suraj; Kumar, Ghantasala S. Sameer; Jacob, Harrys K.C.; Prasad, Thottethodi Subrahmanya Keshava; Mahmood, Riaz; Kumar, K. V. Veerendra; Kumar, M. Vijaya; Meltzer, Stephen J.; Montgomery, Elizabeth A.; Kumar, Rekha V.; Pandey, Akhilesh. 2010. "Overexpression of Periostin and Lumican in Esophageal Squamous Cell Carcinoma." Cancers 2, no. 1: 133-142.


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