Epstein–Barr Virus Gene BARF1 Expression is Regulated by the Epithelial Differentiation Factor ΔNp63α in Undifferentiated Nasopharyngeal Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Plasmids
2.3. In Vitro DNA Methylation
2.4. Luciferase Reporter Assays
2.5. Chromatin Immunoprecipitation (ChIP) Assay
2.6. Quantitative RT-PCR
2.7. SDS-Page and Western Blot
3. Results
3.1. Potential p53 Family Response Elements Found in the BARF1 Promoter
3.2. ΔNp63α, But No Other p53 Family Members, Transactivates BARF1 Promoter in Luciferase Reporter Assays
3.3. ΔNp63α Is Complexed with the BARF1 Promoter In Vivo
3.4. Multiple p63 Response Elements Are Responsible for BARF1 Promoter Transactivation
3.5. ΔNp63α Alone Is Not Sufficient to Induce BARF1 in Context of the Viral Genome
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Conflicts of Interest
References
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ATG-n | Forward primers |
---|---|
679 | CTGACTAGTCTCATCACGCAACACCCACTGTTT |
633 | CTGACTAGTAAGTCAGTCAGGCTGGCCAGG |
582 | CTGACTAGTGATCTTGGCATGCCGCCCAGC |
468 | CTGACTAGTACCGCAAACACCACTGTGTAGC |
410 | CTGACTAGTGGTCGTTGTACACTGCGCGCAG |
350 | CTGACTAGTCGATGTCGGCTGTCCTGCAGG |
327 | CTGACTAGTAGCTCCGCGTACAGCTTCCTATCC |
261 | CTGACTAGTGGCAAAGGCAGGTCTTTCTCATCC |
220 | CTGACTAGTCATGGCCCTGAACATGAGGTAGC |
156 | CTGACTAGTCACGCCTCGACCGGGGTC |
120 | CTGACTAGTGTAGACTTGGCTGGCCTCATGG |
103 | CTGACTAGTCATGGTCTCGTCAGGCCAGC |
63 | CTGACTAGTTGATAAAATGGGCGTGGCAG |
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Share and Cite
Hoebe, E.; Wille, C.; Hagemeier, S.; Kenney, S.; Greijer, A.; Middeldorp, J. Epstein–Barr Virus Gene BARF1 Expression is Regulated by the Epithelial Differentiation Factor ΔNp63α in Undifferentiated Nasopharyngeal Carcinoma. Cancers 2018, 10, 76. https://doi.org/10.3390/cancers10030076
Hoebe E, Wille C, Hagemeier S, Kenney S, Greijer A, Middeldorp J. Epstein–Barr Virus Gene BARF1 Expression is Regulated by the Epithelial Differentiation Factor ΔNp63α in Undifferentiated Nasopharyngeal Carcinoma. Cancers. 2018; 10(3):76. https://doi.org/10.3390/cancers10030076
Chicago/Turabian StyleHoebe, Eveline, Coral Wille, Stacy Hagemeier, Shannon Kenney, Astrid Greijer, and Jaap Middeldorp. 2018. "Epstein–Barr Virus Gene BARF1 Expression is Regulated by the Epithelial Differentiation Factor ΔNp63α in Undifferentiated Nasopharyngeal Carcinoma" Cancers 10, no. 3: 76. https://doi.org/10.3390/cancers10030076