A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood
Abstract
:1. Introduction
2. Materials and Methods
2.1. 3D Printing of the Millifluidic Device
2.2. Bacterial Culture
2.3. Synthesis of Ab-MNPs
2.4. Effect of Flow Rates on Bacteria Capturing Efficiency in the 3DpmFD
2.5. Effect of MSB and Bacterial Concentration on DNA Purification
2.6. Effect of Dilution Factor of Blood on Efficiency of Sample Preparation in 3DpmFD
2.7. Bacterial Preconcentration and DNA Purification in Spiked Blood Samples
2.8. Detection of Bacteria by PCR and qPCR Samples
3. Results and Discussion
3.1. Effect of Flow Rate on Bacteria Capturing Efficiency in the 3DpmFD
3.2. Optimisation of MSB Concentrations for DNA Purification using 3DpmFD
3.3. Effect of Preconcentration and gDNA Purification Using 3DpmFD on Molecular Amplification of Genes in PBS
3.4. Dilution Effect on Preconcentration–Purification Efficiency in Blood Samples
3.5. Spike Test in 10% Blood
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Kim, Y.; Lee, J.; Park, S. A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood. Micromachines 2018, 9, 472. https://doi.org/10.3390/mi9090472
Kim Y, Lee J, Park S. A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood. Micromachines. 2018; 9(9):472. https://doi.org/10.3390/mi9090472
Chicago/Turabian StyleKim, Yonghee, Jinyeop Lee, and Sungsu Park. 2018. "A 3D-Printed Millifluidic Platform Enabling Bacterial Preconcentration and DNA Purification for Molecular Detection of Pathogens in Blood" Micromachines 9, no. 9: 472. https://doi.org/10.3390/mi9090472