Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Microchip Fabrication
2.3. Automation
3. Results and Discussion
3.1. Motion of Fluids in the Loop
3.2. Time Evolution of Droplets
3.3. The Bacterial Growth in Closed Loop Circulation
3.4. Influence of Chloramphenicol on Bacterial Culture
3.5. Verifying the Lack of Cross-Contamination of Tubing Walls during the Continuous Phase
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Debski, P.R.; Sklodowska, K.; Michalski, J.A.; Korczyk, P.M.; Dolata, M.; Jakiela, S. Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures. Micromachines 2018, 9, 469. https://doi.org/10.3390/mi9090469
Debski PR, Sklodowska K, Michalski JA, Korczyk PM, Dolata M, Jakiela S. Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures. Micromachines. 2018; 9(9):469. https://doi.org/10.3390/mi9090469
Chicago/Turabian StyleDebski, Pawel R., Karolina Sklodowska, Jacek A. Michalski, Piotr M. Korczyk, Miroslaw Dolata, and Slawomir Jakiela. 2018. "Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures" Micromachines 9, no. 9: 469. https://doi.org/10.3390/mi9090469