Next Article in Journal
Chiral Orientation of Skeletal Muscle Cells Requires Rigid Substrate
Next Article in Special Issue
Rapid Nucleic Acid Extraction and Purification Using a Miniature Ultrasonic Technique
Previous Article in Journal
Fabrication of Functional Plastic Parts Using Nanostructured Steel Mold Inserts
Previous Article in Special Issue
A Programmable Digital Microfluidic Assay for the Simultaneous Detection of Multiple Anti-Microbial Resistance Genes
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessFeature PaperArticle
Micromachines 2017, 8(6), 180; doi:10.3390/mi8060180

Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT)

1
Thomas Johann Seebeck Department of Electronics, Tallinn University of Technology, Ehitajate Tee 5, 12616 Tallinn, Estonia
2
Selfdiagnostics Deutschland GmbH, 04103 Leipzig, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Nam-Trung Nguyen, Stephen Haswell, Yi Heng Nai and Kirsty Shaw
Received: 24 April 2017 / Revised: 23 May 2017 / Accepted: 1 June 2017 / Published: 7 June 2017
(This article belongs to the Special Issue Application of Microfluidic Methodology for the Analysis of DNA)
View Full-Text   |   Download PDF [1545 KB, uploaded 7 June 2017]   |  

Abstract

Non-instrumented nucleic acid amplification tests (NINAAT) are a novel paradigm in portable molecular diagnostics. They offer the high detection accuracy characteristic of nucleic acid amplification tests (NAAT) in a self-contained device, without the need for any external instrumentation. These Point-of-Care tests typically employ a Lab-on-a-Chip for liquid handling functionality, and perform isothermal nucleic acid amplification protocols that require low power but high accuracy temperature control in a single well-defined temperature range. We propose temperature control solutions based on commercially available heating elements capable of meeting these challenges, as well as demonstrate the process by which such elements can be fitted to a NINAAT system. Self-regulated and thermostat-controlled resistive heating elements were evaluated through experimental characterization as well as thermal analysis using the finite element method (FEM). We demonstrate that the proposed solutions can support various NAAT protocols, as well as demonstrate an optimal solution for the loop-mediated isothermal amplification (LAMP) protocol. Furthermore, we present an Arduino-compatible open-source thermostat developed for NINAAT applications. View Full-Text
Keywords: lab-on-a-chip; LoC; finite element modelling; resistive heating; Point-of-Care; PoC; temperature control; computer aided design; microfluidics; isothermal nucleic acid amplification tests; NINAAT; NAAT lab-on-a-chip; LoC; finite element modelling; resistive heating; Point-of-Care; PoC; temperature control; computer aided design; microfluidics; isothermal nucleic acid amplification tests; NINAAT; NAAT
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Pardy, T.; Rang, T.; Tulp, I. Development of Temperature Control Solutions for Non-Instrumented Nucleic Acid Amplification Tests (NINAAT). Micromachines 2017, 8, 180.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top