Next Article in Journal
Microfluidic Technology for the Generation of Cell Spheroids and Their Applications
Next Article in Special Issue
Ultrahigh-Throughput Improvement and Discovery of Enzymes Using Droplet-Based Microfluidic Screening
Previous Article in Journal
Theoretical and Experimental Study on Vibration Propagation in PMMA Components in Ultrasonic Bonding Process
Previous Article in Special Issue
Microfluidic Production of Multiple Emulsions
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Micromachines 2017, 8(3), 93; doi:10.3390/mi8030093

An Automated Microfluidic System for the Generation of Droplet Interface Bilayer Networks

1
Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland
2
Department of Biophysics, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Andrew J. deMello and Xavier Casadevall i Solvas
Received: 27 January 2017 / Revised: 11 March 2017 / Accepted: 15 March 2017 / Published: 21 March 2017
(This article belongs to the Special Issue Droplet Microfluidics: Techniques and Technologies, Volume II)
View Full-Text   |   Download PDF [2951 KB, uploaded 21 March 2017]   |  

Abstract

Networks of droplets, in which aqueous compartments are separated by lipid bilayers, have shown great potential as a model for biological transmembrane communication. We present a microfluidic system which allows for on-demand generation of droplets that are hydrodynamically locked in a trapping structure. As a result, the system enables the formation of a network of four droplets connected via lipid bilayers and the positions of each droplet in the network can be controlled thanks to automation of microfluidic operations. We perform electrophysiological measurements of ionic currents indicating interactions between nanopores and small molecules to prove the potential of the device in screening of the inhibitors acting on membrane proteins. We also demonstrate, for the first time, a microfluidic droplet interface bilayer (DIB) system in which the testing of inhibitors can be performed without direct contact between the tested sample and the electrodes recording picoampere currents. View Full-Text
Keywords: microfluidics; microdroplets; droplet interface bilayers (DIBs); droplet networks; electrophysiology; model lipid membrane microfluidics; microdroplets; droplet interface bilayers (DIBs); droplet networks; electrophysiology; model lipid membrane
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Czekalska, M.A.; Kaminski, T.S.; Horka, M.; Jakiela, S.; Garstecki, P. An Automated Microfluidic System for the Generation of Droplet Interface Bilayer Networks. Micromachines 2017, 8, 93.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top